微观分析温度对表面殖民的影响和Salmonella enterica的生物膜形态
Zachariah Vice1, You Zhou2, Sapna Chitlapilly Dass1
1Department of Animal Science, Texas A&M University, College Station, TX 77845, USA.
Foods (Basel, Switzerland)
|January 25, 2025
概括
环境温度对肉类接触表面的沙门氏菌 (Salmonella enterica) 生物膜形成有重大影响. 虽然较高的温度促进了强大的生物膜发育,但低温并不能阻止殖民,造成持续的污染风险.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 微生物病原体的产生
背景情况:
- 沙门氏菌 (Salmonella enterica) 是一种重要的食源性病原体,经常污染红肉.
- 在与食品接触的表面上由S. enterica形成的生物膜对标准的消毒方案构成了挑战.
- 环境温度是影响微生物生长和生物膜发育的关键因素.
研究的目的:
- 研究不同温度对沙门氏菌 (Salmonella enterica) 细胞殖民和生物膜形成的影响.
- 想象和比较S. enterica在不同温度下形成的生物膜中的形态差异.
主要方法:
- 使用扫描电子显微镜和共聚焦显微镜.
- 采用新的图像分析软件进行定量评估.
- 在7°C,15°C和37°C的不钢上开发出S. enterica的生物膜.
主要成果:
- 温度升高 (15°C和37°C) 导致生物膜结构更明确,具有显著的细胞外聚合物质 (EPS).
- 即使在低温 (7°C) 条件下也观察到沙门氏体的殖民和生物量.
- 低温限制了EPS生物合成和生物膜成熟,尽管促进了细胞附着.
结论:
- 环境温度是沙门氏菌生物膜发育的关键因素,影响其结构和成熟.
- 在最佳条件下形成的成熟生物膜可以防止日常清洁和消毒.
- 肉类加工厂常见的冷却温度不能完全抑制沙门氏菌的殖民和生物膜的形成.
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