相关实验视频

Updated: May 2, 2026

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

Published on: May 11, 2017

通过LC-MS/MS在发酵过程中产生的三种现场活性维生素B12的同时确定

Zhihao Fan¹, Yajie Li¹, Xia Fan¹

¹Whole Grain Food Engineering Research Center, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

Foods (Basel, Switzerland)

|January 25, 2025

概括

一种新的液体染色学-双重质谱法方法快速量化食品中的活性维生素B12 (VB12) 形式. 这一分析支持开发功能性食品,增添了必不可少的VB12.

科学领域:

食品科学食品科学食品科学
分析化学分析化学
营养生物化学营养生物化学

背景情况:

维生素B12 (VB12) 缺乏是一个全球性的健康问题.
通过发酵在现场强化食品是一种有前途的策略,可以提高VB12的生物可用性.
准确量化VB12形式对于开发强化食品至关重要.

研究的目的:

开发一种快速而准确的液体染色学-并联质谱法 (LC-MS/MS) 方法来量化活性VB12形式.
为了验证该方法对伪VB12的特异性.
在发酵食品矩阵中应用VB12测定方法.

主要方法:

开发一个8分钟的LC-MS/MS方法,用于同时分析腺可巴胺 (AdoCbl),甲基可巴胺 (MeCbl) 和可巴胺 (OHCbl).
提取和净化协议的优化,强调光屏蔽以防止VB12降解.
验证方法应用于发酵的米和Propionibacterium freudenreichii*细胞质量.

主要成果:

开发的LC-MS/MS方法在8分钟内实现了三种活性VB12形式的快速准确量化.
该方法证明了特异性,不受伪VB12的影响.
在样品制备过程中暴露在光线下,发现VB12含量减少了约30%.
在发酵过程中,确定了腺甲胺 (AdoCbl) 是主要的VB12形式.
补充剂没有改变三种分析的VB12形式的比例.

结论:

成功开发了一种新,快速,准确的LC-MS/MS方法,用于同时确定活性VB12形式.
该方法适用于监测功能性食品和发酵产品中的VB12产量.
这些发现为推进食品工业中VB12强化策略提供了关键的分析支持.

关键词:

这是LC-MS/MS.普罗皮奥尼细菌 (Propionibacterium freudenreichii) 是一种常见的细菌.发酵发酵发酵发酵大米子,大米,大米.维生素B12 维生素B12 是一种

更多相关视频

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: January 5, 2021

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...