不同的空化尿样存储时间,温度和防腐剂对多重珠基瘤膀癌免疫检测分析的影响
Sunao Tanaka1, Kaoru Murakami1, Toru Sakatani1
1Samuel Oschin Comprehensive Cancer Center, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.
Diagnostics (Basel, Switzerland)
|January 25, 2025
概括
在室温下储存尿液样本进行Oncuria膀癌症测试会改变生物标志物水平. 冷却 (4°C) 对于精确的尿分析和样本稳定性至关重要,即使使用稳定剂.
科学领域:
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 生物化学 生化学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 尿分析的准确性依赖于稳定的尿样.
- 目前的Oncuria免疫测试需要在4°C的温度下储存,从而增加了实验室的工作量.
- 室温储存可以简化实验室流程并降低成本.
研究的目的:
- 评估尿样采集和储存条件对Oncuria生物标志物分析的影响.
- 为了确定室温储存是否可用于Oncuria测试.
- 为了确定室温尿样保存的潜在稳定剂.
主要方法:
- 尿样在20°C,4°C和-20°C下保存长达一个月.
- 商用尿液稳定剂和抗生素进行了测试.
- 用Oncuria多重免疫试验测量了分析物水平.
主要成果:
- 与4°C储存相比,没有稳定剂的室温储存改变了分析剂水平.
- 商业稳定剂在长时间的室温储存期间无法防止分析物的变化.
- 没有确定合适的稳定剂,以允许室温储存用于Oncuria分析.
结论:
- 在4°C的冷藏是必要的,以防止样品降解,并确保准确的结果.
- 冷藏有利于储存和运输尿液样本进行菌检测.
- 目前的协议要求冷藏的尿样用于可靠的膀癌生物标志物分析.
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