CCL5通过CCR5受体诱导一种类似于sarcopenic的表型
Francisco Aguirre1,2, Franco Tacchi1,2, Mayalen Valero-Breton1,2
1Laboratory of Muscle Pathology, Fragility and Aging, Department of Biological Sciences, Faculty of Life Sciences, Universidad Andres Bello, Santiago 8370146, Chile.
Antioxidants (Basel, Switzerland)
|January 25, 2025
概括
化学基因连接体5 (CCL5) 通过激活CCR5受体,导致肌肉消耗状况的萨尔科佩尼亚. 这项研究揭示了CCL5作为一种新型肌肉激素,有助于肌肉损失和衰弱.
科学领域:
- 肌肉生理学和分子生物学
- 萨尔科佩尼亚研究研究
- 肌肉中的细胞因子信号传递.
背景情况:
- 萨科佩尼亚的特点是肌肉质量和力量的减少.
- 化学激素连接体5 (CCL5) 和它的受体CCR5在sarcopenic肌肉中升高.
- CCL5在诱导肉症中的作用尚不清楚.
研究的目的:
- 调查CCL5和CCR5是否会在骨肌肉中诱导类似于肉症的效应.
- 阐明CCL5诱导的肌肉缩背后的分子机制.
主要方法:
- 过度表达的CCL5在小鼠前 (TA) 肌肉通过电穿孔.
- 用重组CCL5 (rCCL5) 处理C2C12神经管和单独的柔性数字纤维 (FDB).
- 评估肌肉质量,纤维直径,蛋白质含量,E3酶基因表达和氧化应激标志物.
- 使用对抗剂马拉维罗克 (MVC) 对CCR5参与的评估.
主要成果:
- 在TA肌肉中CCL5过度表达引起了sarcopenia,降低了肌肉强度,质量,纤维直径和sarcomeric蛋白,同时上调E3-ligases.
- 在用rCCL5.5处理的培养菌根管和FDB纤维中观察到类似的sarcopenic表型.
- CCL5治疗增加了活性氧物种 (ROS) 和碳化蛋白质的产生,这表明氧化应激.
- 与MVC的CCR5对抗性阻止了rCCL5.5诱导的肉效应.
结论:
- CCL5作为一种新型肌细胞激素,促进一种类似于肉症的表型.
- CCL5的皮效应通过CCR5受体进行介导.
- CCL5诱导的氧化应激会导致肌肉缩.
关键词:
CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5 CCL5CCR5 CCR5 的意思是什么?基因电解是一种基因电解.氧化应激是一种氧化应激.蛋白质合成 蛋白质合成有活性氧物种的反应性氧物种.这种类型的麻症是sarcopenia.无处不在的蛋白酶体系统更多相关视频
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