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在格兰阴性细菌中,种内和种际结合性转移等离子体

Julia R Savelieva¹, Daria A Kondratieva¹, Maria V Golikova¹

¹Department of Pharmacokinetics & Pharmacodynamics, Gause Institute of New Antibiotics, 11 Bolshaya Pirogovskaya Street, 119021 Moscow, Russia.

|January 25, 2025

概括

碳烯酶编码的等离子体从Klebsiella pneumoniae转移到大肠杆菌,增加了美罗胺的耐药性. Pseudomonas aeruginosa 并没有获得这些等离子体. 边界耐药菌株威胁到抗生素的有效性.

科学领域:

微生物学微生物学
分子生物学分子生物学
药理学药理学是指药理学的学科.

背景情况:

通过等离子体介导的耐药性显著降低了抗生素的有效性,特别是卡巴.
碳烯酶编码等离子体是抗菌素耐药性的主要驱动因素.
了解等离子体转移对于打击抗生素耐药性至关重要.

研究的目的:

为了确定从Klebsiella pneumoniae转移到Escherichia coli和Pseudomonas aeruginosa的碳烯酶编码等离子体的频率.
评估等离子体获取对美罗胺最小抑制度 (MICs) 的影响.
为了评估在梅罗胺压力下耐美罗胺耐药大肠杆菌转结合物的体外活力.

主要方法:

对细菌的结合实验 (交配) 在上进行.
使用PCR.隔离和识别转结合体,受体和捐赠体殖民地.
确定美罗胺MIC和结合频率 (CF).
使用空洞纤维感染模型进行药理动力学评估.

主要成果:

肺炎和大肠杆菌之间的结合频率 (CF) 与物种内部转移相似.
与接受者 (0.03-0.06μg/mL) 相比,K. pneumoniae和E. coli转合剂 (0.25-4μg/mL) 的梅罗胺MIC增加.
P. aeruginosa没有从K. pneumoniae获得等离子体.
在药理动力学研究中,一种具有边界美罗酶敏感性的大肠杆菌转合剂 (MIC 2 mg/L) 没有对治疗产生反应.

结论:

肺炎和大肠杆菌之间的结合频率是可比的.
在同一物种内,K. pneumoniae对等离子体的获取具有较高的允许性.
在K. pneumoniae和P. aeruginosa之间没有观察到等离子体的转移.
具有边界梅罗胺敏感性的转结合剂对卡巴胺疗效构成重大威胁.

关键词:

埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.克莱布西拉肺炎是一种肺炎.伪omonas空气菌伪omonas空气菌碳烯的质量非常大.结合方式结合方式虚空纤维感染模型的模型.更多的是openemenem.

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