复星通过恢复线粒体功能来缓解牛乳腺上皮细胞中NEFA诱导的氧化损伤
Longwei Sun1,2, Junpeng Huang1,2, Xiangyang Dou1,2
1Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, China.
Animals : an open access journal from MDPI
|January 25, 2025
概括
复星 (RES) 保护牛乳细胞免受高非化脂肪酸 (NEFAs) 引起的氧化损伤. 它增强了抗氧化剂防御,并恢复了线粒体功能,为奶牛的代谢问题提供了潜在的治疗方法.
科学领域:
- 生物化学 生化学
- 动物科学动物科学
- 细胞生物学 细胞生物学
背景情况:
- 产前乳牛经历负能量平衡,导致高非化脂肪酸 (NEFAs).
- 尼法诱导氧化应激和代谢功能障碍,对乳制品行业构成挑战.
- 复星 (RES) 是一种多,具有已知的抗氧化和抗炎性质.
研究的目的:
- 研究 RES 对 NEFA 挑战的牛乳腺上皮细胞 (BMEC) 的抗氧化作用.
- 阐明 RES 在缓解 NEFA 诱导的氧化损伤方面的潜在分子机制.
- 用小鼠模型在体内评估RES的疗效.
主要方法:
- 用NEFAs对BMEC进行了挑战,并用RES进行了处理.
- 评估了氧化应激标志物 (甲,活性氧物种).
- 评估了线粒体功能 (膜潜力,ATP产生,NAD+/NADH比) 和亡.
- 使用高脂肪饮食诱导的氧化损伤小鼠模型进行的体内研究.
主要成果:
- 通过提高抗氧化酶的调节和减少甲和活性氧物种,RES处理减少了NEFA诱导的氧化损伤.
- RES恢复了线粒体功能,增加了线粒体膜潜力,ATP生产和NAD+/NADH比率,同时减少了亡.
- 在体内研究表明,RES在小鼠乳腺组织中减轻了氧化损伤和线粒体功能障碍.
结论:
- ресвератрол有效地改善牛乳腺上皮细胞中NEFA诱导的氧化损伤和线粒体功能障碍.
- RES调节线粒体功能,为具有负能量平衡的奶牛的氧化应激提供了潜在的治疗策略.
- 这些发现支持RES作为一种预防和治疗药物,用于对付周围乳牛的代谢障碍.
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