缓解Clostridium perfringens诱导的肠病变,使用XylanaseCbXyn10C及其与蛋白酶的二元尾酒
Wenjing Zhang1, Zhenzhen Hao1, Daoxin Yang1
1State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Animals : an open access journal from MDPI
|January 25, 2025
概括
杆菌的感染会在中引起死肠炎. 用西兰酶和性蛋白酶补充料显著减少了肠道损伤,通过增强屏障基因和有益细菌,改善了肠道健康.
科学领域:
- 动物科学动物科学
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 杆菌的感染导致肉死肠炎,造成严重的经济损失.
- 像西兰酶和蛋白酶这样的酶在管理肠道健康和对抗细菌感染方面表现出潜力.
研究的目的:
- 为了评估一种西兰酶 (CbXyn10C) 和一种蛋白酶在减轻肉中Clostridium perfringens诱导的死肠炎的疗效.
- 评估酶补充对肠道完整性,炎症反应和肠道微生物群的影响.
主要方法:
- 300只雄性AA被分为五组:对照组,仅对C. perfringens进行挑战,以及对C. perfringens加上不同酶组合 (xylanase,xylanase+amylase,xylanase+alkaline protease) 的挑战.
- 从第14天到第20天,C. perfringens是口服的. 分析了肠道损伤,基因表达,细胞因子水平 (IL-6),二胺氧化酶 (DAO) 水平,以及肠道细菌群 (乳杆菌,菌).
主要成果:
- 与C. perfringens挑战组相比,西兰酶和性蛋白酶 (Xyn+Ap) 的组合显著降低了十二指肠,阴肠和阴肠的肠损伤 (p <0.05).
- 酶补充,特别是Xyn+Ap,增强了肠道屏障基因的表达,降低了IL-6水平,降低了DAO水平.
- 酵素养对肠中的乳酸菌和乳酸菌细菌的丰富程度产生了积极的影响.
结论:
- 克西兰酶 (CbXyn10C) 和蛋白酶有效地缓解了肉中Clostridium perfringens感染引起的肠道损伤.
- 酶补充可以改善肠道屏障功能,调节炎症反应,促进有益的肠道微生物群,为治疗死肠炎提供了可行的策略.
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