在中国,从驴子便中获取乳酸细菌的益生菌特征
Yanqiu Wu1,2,3, Shousong Yue1,2,3, Jinhui Yu1,2,3
1Institute of Crop Germplasm Resources, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Animals : an open access journal from MDPI
|January 25, 2025
概括
研究人员从驴便中选乳酸细菌 (LAB) 来检测益生菌的潜力. 一个分离物Ligilactobacillus salivarius L9显示出有希望的抗微生物和生存特征,可用于驴.
科学领域:
- 微生物学 微生物学
- 动物科学动物科学
- 益生菌研究 益生菌研究
背景情况:
- 益生菌为动物提供健康益处,包括免疫调节和肠道健康.
- 在中国,驴子养殖正在增长,增加了对有效驴子特异性益生菌的需求.
- 从驴子中分离出来的益生菌菌株的研究是有限的.
研究的目的:
- 从驴便中选乳酸细菌 (LAB),以检测它们的益生菌性质.
- 评估孤立的LAB菌株的抗菌活性和体外益生菌特性.
主要方法:
- 从驴便中分离和选LAB.
- 对致病细菌 (大肠杆菌,黄金杆菌,型杆菌,S. abortus equi) 的抗菌活性评估.
- 评估LAB对低pH值,胆盐和人工胃/肠液的耐受性.
- 分析自身聚合性,疏水性和协聚性能的分析.
- 使用分子方法识别有前途的分离物.
主要成果:
- 十三个LAB分离体对测试的病原体表现出抗微生物活性;八个抑制了所有四个.
- 七个分离体对低pH值 (>50%的存活率) 和胆盐 (>60%的存活率) 具有良好的耐受性.
- 五个分离物能够在人工胃和肠液中存活 (>50%的存活率).
- 三个分离物体显示出良好的自聚 (>40%),疏水性 (>40%),和协聚.
- 鉴定出 Ligilactobacillus salivarius L9 是一个有前途的候选人,符合体外益生菌标准.
结论:
- 该研究成功地从驴便中确定了潜在的益生菌LAB菌株.
- 利吉拉克托巴西勒斯 (Ligilactobacillus salivarius L9) 在体外表现出显著的益生菌特征,包括抗菌活性和耐压力.
- L. salivarius L9 是开发益生菌来改善驴子健康和表现的潜在候选者.
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