草的病理和分子特征与两个Aeromonas物种共同感染
Wenyao Lv1,2,3, Zhijie Zhou1,2,3, Lingli Xie1,2,3
1Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China.
Animals : an open access journal from MDPI
|January 25, 2025
概括
与Aeromonas hydrophila和Aeromonas veronii同时感染的草发生了高死亡率和严重的肝损伤. 免疫反应被激活,而代谢途径被抑制,为鱼类疾病提供了洞察力.
科学领域:
- 水生动物免疫学
- 鱼类病理学 鱼类病理学
- 微生物的病原发生.
背景情况:
- 草鱼 (Ctenopharyngodon idella) 容易受到Aeromonas物种的感染.
- 与Aeromonas hydrophila和Aeromonas veronii共同感染期间的免疫机制尚未得到充分理解.
研究的目的:
- 在同时感染Aeromonas hydrophila和Aeromonas veronii期间,研究草的病原和宿主免疫反应.
主要方法:
- 草被A.hydrophila和A.veronii的共同感染.
- 肝脏组织的细菌负荷,组织病理学和比较转录学分析在感染后的1天和5天 (dpi).
- 对差异表达基因 (DEGs) 进行了基因本体学和KEGG通路分析.
主要成果:
- 同时感染导致显著的死亡率 (68%) 和肝脏中的细菌负载增加3dpi.
- 组织病理学揭示了严重的肝病变,包括缩和出血.
- 转录组分析分别发现了1dpi和5dpi的868和411个DEG,在Toll样受体和TNF信号通路中显著丰富.
- 代谢途径被抑制,而免疫反应被激活.
结论:
- 同时的Aeromonas感染会导致草的严重病原和显著的免疫激活.
- 了解这些宿主-病原体相互作用可以为水产养殖中的疾病预防和控制策略提供信息.
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