关于Clostridium perfringens菌体Endolysin PlyDolk2121的表征 菌体Endolysin PlyDolk21的表征
Suyoung Seo1, Bokyung Son2, Minsuk Kong1
1Department of Food Science and Biotechnology, Research Institute of Food and Biotechnology, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea.
Antibiotics (Basel, Switzerland)
|January 25, 2025
概括
一种新型的内素,PlyDolk21,有效地向食品中的抗生素耐药的Clostridium perfringens菌株. 这种细菌衍生的蛋白质显示出作为生物控制剂和食品安全检测工具的前景.
科学领域:
- 食品微生物学 食品微生物学
- 细菌生物学的生物学
- 抗菌剂 抗菌剂 抗菌剂
背景情况:
- 杆菌是导致食物传播疾病的主要原因之一.
- 在C. perfringens的抗生素耐药性需要替代的控制方法.
- 菌体及其衍生蛋白质提供了潜在的解决方案.
研究的目的:
- 隔离和描述一个菌体 (Dolk21) 和它的内溶素 (PlyDolk21) 向C. perfringens.
- 评估PlyDolk21及其细胞壁结合域 (CBD) 的抗菌和结合活性.
- 评估PlyDolk21在减少C. perfringens在食品矩阵中的有效性.
主要方法:
- 细菌菌体Dolk21和内素PlyDolk21的分离和表征.
- 对PlyDolk21对C. perfringens菌株的催化活性进行评估.
- 在体外和食品样本 (牛肉,牛奶) 中评估PlyDolk21及其CBD的抗菌和结合活性.
主要成果:
- PlyDolk21对所有测试的C. perfringens菌株表现出性活性,与其特定的菌体不同.
- 与其催化域相比,全长的PlyDolk21表现出增强的催化活性.
- 在食品中,PlyDolk21_CBD成功地与C.perfringens结合,PlyDolk21在牛肉和牛奶中减少了3-log的细菌数量.
结论:
- 普利多尔克21是一种强大的抗菌剂,用于对抗C. perfringens.
- 普利多尔克21及其CBD显示了生物控制和检测C.perfringens在食品中的显著潜力.
- 这种内素代表了食品安全应用中抗生素的有希望的替代品.
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