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在无酸的上从天然草地上分离的细菌产生了许多没有基酶的菌株

Amrit Koirala¹, Nabilah Ali Alshibli¹, Bikram K Das¹

¹Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA.

|January 25, 2025

概括

提高生物固是可持续农业的关键. 用于分离各种固定的细菌的新方法揭示了许多分离物,包括像Bacillus这样的常见属,对酶基因 (nifH) 没有阳性测试.

科学领域:

微生物学微生物学
农业科学农业科学
生物技术是生物技术.

背景情况:

可持续的作物生产需要通过生物勘探有效的固细菌来加强生物固 (BNF).
使用低氧条件的传统隔离方法通常只能产生最具竞争力的菌株,从而限制微生物多样性.
在氧气减少的环境中在固体介质上涂抹可以增加可培养的二氧化菌细菌的多样性.

研究的目的:

从天然草原土壤样本中分离出各种固定的细菌.
用严格的无介质和酶基因 (nifH) 的PCR放大来确认固能力.
评估在没有的介质上生长的可靠性,以识别固定细菌.

主要方法:

来自27个草原样本的土壤悬浮被涂在固体无酸盐上.
在大气和减少氧气的条件下进行了化.
假定固定剂通过在无液介质中进行亚培养和nifH基因的PCR放大来确认;分离物进一步在严格的无介质和贫大气中进行了测试.

主要成果:

在442个分离物中,只有17%产生了nifH基因的amplicon,这表明确认固化的成功率很低.
许多最初似乎在没有的介质上固定的分离物被发现是共同培养物,只有一个成员在限制下生长.
常见的土壤细菌,如Bacillus和Streptomyces,这些细菌是nifH PCR阴性,经常被隔离,这表明潜在的替代获取或新减少系统.

结论:

隔离和确认固细菌的标准方法可能会高估它们的流行率和多样性.
建议在评估固化时,即使在严格的条件下,也应谨慎,仅基于无介质的生长.
需要进一步的研究来了解nifH阴性分离物的代谢,特别是来自Bacillus和Streptomyces属的分离物.

关键词:

有文化的,有文化的,有文化的.这种体质的体质是:diazotroph.固定的方法是固定.化酶是一种化酶.草原是一个草原.土壤土壤土壤土壤土壤

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