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日常使用的厨房毛巾上的生物膜成分和细菌多样性

Yao Zhang¹, Kexin Li¹, Yibo Ru¹

¹School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.

|January 25, 2025

概括

厨房毛巾含有大量的细菌生物膜,主要由蛋白质,多糖和eDNA组成. 像Kocuria和Pseudomonas这样的占主导地位的细菌可能来自食物和人与人接触,突出了卫生问题.

科学领域:

微生物学微生物学
材料科学材料科学材料科学

背景情况:

复杂的编织毛巾结构容易形成生物膜.
在现实条件下,厨房毛巾生物膜的微生物组成在很大程度上是未知的.

研究的目的:

为了研究生物膜发育,细菌组成和厨房毛巾的物理变化,每天使用10周.
分析环境因素,细菌属和毛巾特性之间的相关性.

主要方法:

12名志愿者在厨房中使用的毛巾被分析了颜色,结构完整性和生物膜的存在.
细菌度被量化 (日志CFU/g).
分析了细胞外聚合物质 (EPSs),并使用高通量测序确定了细菌多样性.

主要成果:

所有使用的毛巾都显示出含有细菌和EPSs的可见生物膜.
细菌度在4至7log CFU/g之间.
蛋白质是最丰富的EPS,其次是多糖和eDNA. 主要属包括Kocuria,Rothia,Psychrobacter,Enhydrobacter和Pseudomonas,它们可能源于食物和人与人接触.

结论:

厨房毛巾积累了各种细菌生物膜,具有显著的EPS含量.
细菌群体是可变的,但包括与食物和人类环境相关的属.
了解这些因素为改善毛巾卫生实践提供了基础.

关键词:

细菌多样性的细菌多样性生物膜是一种生物膜.高通量测序的高通量测序毛巾毛巾毛巾

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