擦拭剂,滴水,还是肉? 在真空包装生牛肉中微生物采样方法的比较
Aracely Martínez-Moreno1, America Chávez-Martínez1, Janet E Corry2
1UACH-CA03 Tecnología de Alimentos de Origen Animal, Facultad de Zootecnia y Ecología, Universidad Autónoma de Chihuahua, Periférico Fco. R. Almada, Chihuahua 33820, Mexico.
Microorganisms
|January 25, 2025
概括
滴滴采样方法从真空包装的牛肉中恢复的微生物,包括乳酸细菌 (LAB) 和沙门氏菌,相比于切割或擦拭. 这种方法是推用于准确的肉制品微生物计数.
科学领域:
- 食品微生物学 食品微生物学
- 微生物采样技术 微生物采样技术
- 肉类科学 肉科学
背景情况:
- 准确的微生物检测对于食品安全和质量评估至关重要.
- 存在各种方法来列举食品中的微生物,但它们的有效性可能会有所不同.
- 真空包装是一种常见的肉类保存方法,影响微生物生长和检测.
研究的目的:
- 为了比较三种采样方法的微生物回收效率:滴滴,切割和擦拭.
- 根据真空包装牛肉中特定指标生物体的可活性计数来评估方法.
- 为了确定最有效的采样方法,在这个食品矩阵中进行微生物计数.
主要方法:
- 在真空包装的牛肉样本中,采用了三种采样方法 (滴滴,切割,擦拭).
- 微生物的数目集中在可行的Enterobacteriaceae,乳酸细菌 (LAB),Brochrothrix thermosphacta,Salmonella spp.,和酵母和菌 (Y&M) 的数量上.
- 统计分析 (p < 0.05) 用于比较方法之间的微生物恢复率.
主要成果:
- 滴滴方法为B. thermosphacta,Salmonella spp.,Enterobacteriaceae,LAB和Y&M产生了明显更高的计数,而不是切割和抽取 (p <0.05).
- 切割显示B.热和肠杆菌类的恢复率高于抽取 (p < 0.05).
- 在除和抽取沙门氏菌spp.,LAB和Y&M之间没有发现显著差异 (p > 0.05).
结论:
- 滴滴采样方法对于从真空包装牛肉中回收更广泛的微生物来说是优越的.
- 这种方法可以产生高达2个对数的微生物回收比切割或擦拭.
- 滴滴方法应优先考虑肉类行业的采样策略,以确保准确的微生物学评估.
更多相关视频
05:34Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products
Published on: February 23, 2024
1.7K
08:19Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products
Published on: January 31, 2025
310
相关概念视频
01:32Tissue Homogenization and Cell Lysis
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
01:26Physical Methods for Controlling Microbial Growth: Radiation and Filtration
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.