相关实验视频

Updated: May 31, 2025

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Published on: November 23, 2017

泌尿酸酶等离子素激活系统在转变细胞系中的调节

Diana Culej Bošnjak¹, Tihana Balent¹, Petra Korać¹

¹Department of Biology, Faculty of Science, University of Zagreb, Horvatovac 102, 10000 Zagreb, Croatia.

International journal of molecular sciences

|January 25, 2025

概括

泌尿酸酶等离子体激活系统 (uPA) 调节细胞行为. 操纵其组成部分,尿激酶等离子体激活剂 (uPA) 和等离子体激活剂抑制剂-1 (PAI1),改变了细胞迁移和球形形成.

科学领域:

生物化学生物化学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

等离子体激活系统 (PAS) 调节细胞外蛋白酶等离子体活性.
PAS的组成部分包括尿素酶等离子激素激活剂 (uPA),其抑制剂PAI1和受体PAR.
在组织重塑和细胞外基质降解中,PAS至关重要,影响生理和病理过程.

研究的目的:

研究uPA,PAI1和UPAR在细胞过程中的作用.
分析这些PAS成分在质母细胞瘤和HEK 293细胞中的过度表达的影响.

主要方法:

在A1235和HEK 293细胞系中克隆和过度表达人类uPA,PAI1和UPAR.
分析尿素激酶活性,蛋白质和RNA表达通过西部斑块和RTqPCR.
细胞增殖,迁移,入侵,粘附和分散的评估,使用细胞计数,伤口愈合,穿孔和球形形成试验.

主要成果:

与uPA的传染增加了尿激酶活性和uPA的表达.
PAI1 传染减少了尿素酶活性,增加了 PAI1 表达,减少了细胞迁移.
HEK 293表达PAI的细胞形成了较小的球形.

结论:

uPA系统分子的影响是相互依赖的,并受到微环境的影响.
细胞类型特定的信号传输在观察到的 uPA 系统效果中发挥着作用.

关键词:

粘附性粘附性粘附性粘附性移民移民迁移迁移塑原激活剂抑制剂抑制剂它们的扩散和扩散.泌尿酸酶等离子体激活剂激活剂

更多相关视频

An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus

An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus

Published on: August 25, 2017

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors

Published on: July 17, 2020

相关概念视频

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...