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冷等离子体对生物膜形成的活性假肢关节感染病原体

Christopher Spiegel¹, Débora C Coraça-Huber¹, Michael Nogler²

¹Research Laboratory for Biofilms and Implant Associated Infections (BIOFILM LAB), Experimental Orthopaedics, University Hospital for Orthopaedics and Traumatology, Medical University of Innsbruck, Müllerstraße 44, 6020 Innsbruck, Austria.

Pathogens (Basel, Switzerland)

|January 25, 2025

概括

冷大气等离子体 (CAP) 有效地减少医疗植入物上的细菌. 在5秒内1厘米的空气等离子体显示了预防周围假肢关节感染的最佳结果.

科学领域:

生物医学工程生物医学工程
传染性疾病传染性疾病
等离子体物理学的物理学

背景情况:

周围假肢关节感染 (PJIs) 影响了手术后的1-2%的患者.
传统的治疗方法,如分阶段修订,在减少PJI方面取得了有限的成功.
冷大气等离子体 (CAP) 显示了表面细菌减少的潜力.

研究的目的:

评估CAP在降低金属表面细菌负荷方面的有效性.
为了研究不同距离和等离子组合 (空气与) 的影响.
为了确保血应用的温度低于体内使用有害的温度.

主要方法:

在TMZF磁盘上使用*金黄色葡萄球菌*和*表皮葡萄球菌*形成生物膜.
用空气和等离子体在不同距离和持续时间进行CAP处理.
在血应用过程中监测温度.
殖民地形成单位 (CFU) 计数以评估细菌减少.

主要成果:

空气和等离子体都在1厘米的距离上表现出有效性.
空气等离子体实现了更高的CFU减少,但由于氧气诱导的温度升高而面临限制.
阿贡血没有表现出与暴露时间相关的温度限制.
最佳条件:1厘米的距离,5秒暴露在空气等离子体中,有效地减少细菌而不会损害组织温度.

结论:

在特定条件下,CAP,特别是空气等离子体,是减少植入物上的细菌污染的可行选择.
阿贡等离子为CAP在手术环境中的应用提供了一个温度稳定的替代方案.
对优化CAP参数的进一步研究对于临床PJI预防是有必要的.

关键词:

生物膜是一种生物膜.寒冷的大气等离子体是什么?骨科骨科医生骨科医生假肢关节感染假肢关节感染葡萄糖球菌 (Staphylococci) 是一种葡萄糖球菌.

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