利用单细胞多基因组来解码瘤微环境多样性和治疗阻力
Hussein Sabit1, Borros Arneth2, Timothy M Pawlik3
1Department of Medical Biotechnology, College of Biotechnology, Misr University for Science and Technology, P.O. Box 77, Giza 3237101, Egypt.
Pharmaceuticals (Basel, Switzerland)
|January 25, 2025
概括
单细胞多组学揭示了瘤微环境 (TME) 的复杂性,揭示了免疫逃避和抵抗机制. 这加深了对癌症进展的理解,并通过分析细胞和分子异质性来指导个性化疗法.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 单细胞多组技术使瘤微环境 (TME) 的详细分析成为可能.
- 了解TME异质性对于癌症进展和治疗耐药性至关重要.
- 细胞的关键组成部分包括癌细胞,癌症相关纤维细胞 (CAF),免疫细胞和内皮细胞 (EC).
研究的目的:
- 审查单细胞多组学如何促进对TME异质性的理解.
- 突出了解免疫逃避和治疗耐药性机制的见解.
- 引导个性化癌症疗法的开发.
主要方法:
- 在单细胞分辨率下整合基因组,转录基因组,蛋白质基因组和代谢基因组数据.
- 在TME中分析细胞和分子复杂性.
- 检查癌细胞,CAF,免疫细胞和EC之间的相互作用.
主要成果:
- 在TME中识别多种细胞类型及其功能异质性.
- 发现了免疫逃避机制,如T细胞疲劳和代谢重编程.
- 揭示了包括 stromal 因素和细胞外矩阵障碍在内的抵抗机制.
结论:
- 单细胞多组学提供了对TME多样性及其对瘤行为的影响的关键见解.
- 了解TME异质性是克服治疗耐药性的关键.
- 以代谢途径和空间TME数据为重点的未来研究将增强个性化癌症治疗.
相关概念视频
02:17The Tumor Microenvironment
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
02:17The Tumor Microenvironment
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...