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Published on: October 19, 2013
在产前缺氧后使用NO调节剂对内皮功能障碍的药物纠正的可能性
Igor Belenichev1, Olena Popazova2,3, Oleh Yadlovskyi3
1Department of Pharmacology and Medical Formulation with Course of Normal Physiology, Zaporizhzhia State Medical and Pharmaceutical University, 69000 Zaporizhzhia, Ukraine.
产前缺氧会导致内皮功能障碍和长期的心血管问题. 安吉奥林和蒂奥特里亚佐林在小鼠幼中有效地逆转了这些影响,突出了它们的治疗潜力.
科学领域:
- 心血管科学 心血管科学
- 新生儿生理学 新生儿生理学
- 内皮细胞生物学 内皮细胞生物学
背景情况:
- 产前缺氧 (PH) 是发展长期心血管疾病的重要危险因素.
- 内皮功能障碍 (ED),通常与氧化 (NO) 缺乏有关,是PH诱导的心血管问题的基础的主要机制.
- NO调节剂对与PH相关的心血管并发症具有潜在的治疗途径.
研究的目的:
- 研究NO调节剂在缓解内皮功能障碍和产前缺氧引起的心血管变化的疗效.
- 评估蒂奥特里亚佐林和安吉奥林对暴露于PH的后代内皮和心脏参数的保护作用.
主要方法:
- 怀孕的老鼠通过化给药暴露于缺氧;后代在产后接受盐水,安吉奥林或提奥特里亚佐林治疗.
- 使用ELISA在心脏细胞质中评估sEPCR,Tie2,VEGF-B,SOD1,GPX1和GPX4的水平.
- 通过实时PCR分析了VEGF和VEGF-BmRNA表达,并通过形态测量评估了心肌微血管内皮细胞核区域.
主要成果:
- 安吉奥林和提奥特林显著改善了PH诱导的内皮功能障碍.
- 这些药物降低了可溶性内皮蛋白C受体 (sEPCR) 和增加了Tie2氨酸激酶,VEGF-B和VEGF-BmRNA.
- 治疗还提高了Cu/ZnSOD和GPX水平,并增加了PH受影响的小鼠幼鼠内皮细胞的核面积.
结论:
- 用NO调节器进行早期产后心脏和内皮细胞保护对于患有PH的新生儿至关重要.
- 在PH之后的心血管疾病的发病过程中,NO依赖机制起着至关重要的作用.
- 安吉奥林和蒂奥特里亚佐林在治疗PH引起的心血管并发症方面具有显著的治疗潜力.
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