饮食纤维和多在孕前,孕期或哺乳期对肠道基因表达的影响
Daniela Ceballos-Sánchez1,2, Laura Sáez-Fuertes1,2, Sergi Casanova-Crespo1,2
1Physiology Section, Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Science, University of Barcelona (UB), 08028 Barcelona, Spain.
Nutrients
|January 25, 2025
概括
在前孕期,孕期或哺乳期的老鼠中,富含纤维和多 (HFP) 的饮食显著改变了小肠基因表达. 越接近分析的HFP饮食,基因调制就越大,影响免疫信号和细胞生长.
科学领域:
- 营养科学 营养科学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 饮食成分在孕前,孕期和哺乳期对母亲和后代的健康产生重大影响.
- 地中海饮食,富含纤维和多,与众多的健康益处有关.
- 了解敏感生命阶段特定饮食模式的分子效应至关重要.
研究的目的:
- 为了研究高纤维,高聚 (HFP) 饮食对大鼠小肠基因表达的影响.
- 为了确定HFP饮食暴露的时间 (孕前,孕期或哺乳期) 是否影响基因表达结果.
- 在关键的生殖期内识别受HFP饮食影响的特定基因和通路.
主要方法:
- 在孕前期 (P),孕期 (G) 或哺乳期 (S) 中,老鼠被食参考饮食或HFP饮食三周.
- 小肠的基因表达用mRNA测序 (HiSeq X-Ten Illumina) 进行分析,并通过实时PCR和ELISA证实.
- 分析的重点是基因调制与HFP饮食干预的时间相关.
主要成果:
- 这种HFP饮食显著调节了大鼠小肠中广泛的基因.
- 当HFP饮食更接近分析时间点 (S > G > P) 时,基因调制更为明显.
- 受影响的基因主要涉及免疫信号,肠道吸收和细胞生长,包括费里丁和脂肪酸合成酶等关键基因. 一个独特的基因Fam117A在所有干预期内都被修改了.
结论:
- 在出生前三周的HFP饮食干预可以显著改变大鼠的肠道基因表达.
- 这些由饮食引起的基因表达变化可以影响母亲的健康,并间接影响后代的发育.
- 饮食干预的时间在基因表达调节的程度上起着作用,突出了营养影响的关键窗口.
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