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使用叶子,根和花朵优化Haworthia truncata Schönland在体外传播

Leila Soleimani¹, Hassan Salehi¹, Taras Pasternak²

¹Department of Horticultural Science, School of Agriculture, Shiraz University, Shiraz 7144113131, Iran.

Plants (Basel, Switzerland)

|January 25, 2025

概括

由于其稀缺性,通过组织培养对Haworthia truncata的大规模传播至关重要. 这项研究优化了植物生长调节器和扩散型,以有效地再生这种独特的果汁植物.

科学领域:

植物生物技术植物生物技术
园艺科学园艺科学
植物生理学植物生理学

背景情况:

南非本土的Haworthia truncata面临着有限的生长和稀缺性,导致高生产成本.
全球对Haworthia物种的贸易非常重要,中国和土耳其是主要的出口国.
组织培养是一种可行的方法,可以大量传播像H. truncata. truncata. 这样的可取的果实植物物种.

研究的目的:

优化组织培养协议,以有效地进行Haworthia truncata的大规模传播.
评估不同植物生长调节剂 (IBA,NAA,BA) 对诱导和芽繁殖的影响.
确定再生最有效的开拓物种类型 (叶子,根,花朵).

主要方法:

穆拉希奇和斯库格 (MS) 基底介质补充了不同度的英多尔-3-黄油酸 (IBA),纳乙烯酸 (NAA) 和氨酸 (BA).
包括叶子,根和花朵在内的植被被培养在具有不同植物生长调节剂 (PGR) 或没有PGR (控制) 的介质上.
评估了诱导,芽生殖和根植的百分比,以确定最佳的再生条件.

主要成果:

开花式开花体显示出最高的诱导 (1.5 mg/L IBA) 和射击增殖 (1 mg/L IBA).
叶子拓展者在0.05 mg/L的NAA和0.25 mg/L的BA中表现最好,用于诱导,0.05 mg/L的NAA和1 mg/L的BA用于芽生殖.
根拓展剂实现了最大的诱导 (0.25 mg/L BA和 0.25 mg/L NAA) 和芽繁殖 (0.05 mg/L NAA和 1 mg/L BA).
最高的再生芽的根植百分比是在1⁄2 MS介质上获得的,并补充了1.5 mg/L的IBA.

结论:

植物生长调节剂和扩增剂类型的特定组合显著影响Haworthia truncata再生的效率.
使用选定的PGR和扩展剂的优化组织培养协议可以促进Haworthia truncata的大规模传播.
这项研究为商业规模生产Haworthia truncata提供了基础,解决了稀缺性和高成本问题.

关键词:

这就是SEM SEM.适应和适应的过程偶然的发芽再生的再生过程微传播是一种微传播.果汁的植物果汁的植物.组织培养的组织培养.

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