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关于黑色科霍什 (Actaea racemosa L.) 中生物活性胺的生物合成

Martin J Spiering¹, James F Parsons¹, Edward Eisenstein^1,2

¹Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD 20850, USA.

Plants (Basel, Switzerland)

|January 25, 2025

概括

黑色科霍什 (Actaea racemosa) 含有三聚二甲酸脱酶 (TDC) 酶,可以产生胺,这是血清素的前体. 较高的TDC活性对于这种植物的血清素生产至关重要.

科学领域:

植物生物化学和分子生物学
植物化学植物化学
药理学是指药理学,即药理学是指药理学.

背景情况:

植物补充剂,如黑 (Actaea racemosa) 具有复杂的作用机制和安全问题.
生物活性模拟物N-甲基色胺存在于A. racemosa中,并有助于其色胺活性.
假设三脱氧酶 (TDC) 酶可以将L-三转化为三胺,这是血清素前体.

研究的目的:

为了研究黑TDC类基因在胺和胺生物合成中的作用.
描述黑TDCs的活性及其对血清激素化合物生产的贡献.
在A. racemosa. 中识别和量化血清衍生物.

主要方法:

在Saccharomyces cerevisiae中克隆和表达黑TDC基因 (TDC1,TDC2,TDC3).
在酵母中与三胺5-基酶 (T5H) 共同表达TDC,以评估血清素的产量.
位点定向突变发生,以确定TDC活性位点中的关键残留物.
使用LC-MS进行植物化学分析,以检测和量化A. racemosa组织中的胺.
用三胺和N-甲基三胺对A. racemosa叶组织进行化.

主要成果:

在酵母产生的氨酸中表达黑色TDC1,TDC2和TDC3.
与TDC2相比,TDC1和TDC3的活性较高,与可变活性部位残留物有关.
高活性TDC与大米T5H的同时表达产生了血清素,表明TDC活性是限制速度的步骤.
在LC-MS分析中,在A. racemosa.中发现了三胺,血清素,N-甲基血清素和布福因.
化实验表明,甲基化在N-甲基胺生物合成中先于化.

结论:

黑TDC酶在胺和随后的胺化合物的生物合成中发挥着关键作用.
在A. racemosa.中,TDCs的活性是产生血清素及其衍生物的关键决定因素.
黑色中存在比以前知道的更多种类型的血清素衍生物,包括潜在的有毒布福因,比以前知道的更多.

关键词:

它们中的一种是Actaea racemosa.在Cimicifuga的比赛中,我们得到了胜利.这种植物属于Rannunculaceae.黑色的科霍什 (Cohosh) 是一个黑色的科霍什 (Cohosh).二次新陈代谢二次新陈代谢血清胺 (serotonin) 是一种神经元.试胺类药物可以托芬脱碳酶酶的作用是什么

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