功能性碳水化合物活性酶通过横向基因转移从白贝米西亚塔巴西植物中获得的功能性碳水化合物活性酶
Dominique Colinet1, Mireille Haon2,3, Elodie Drula2,4
1Institut Sophia Agrobiotech, INRAE, Université Côte d'Azur, CNRS, Sophia Antipolis, France.
Genome biology and evolution
|January 25, 2025
概括
白Bemisia tabaci通过横向基因转移获得了植物性碳水化合物活性酶,包括新型β-葡萄糖酶. 这一发现揭示了植物食昆虫在碳水化合物同化方面的独特适应性.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 昆虫的生物化学 昆虫的生物化学
背景情况:
- 碳水化合物活性酶 (CAZymes) 对于分解植物细胞壁和利用碳水化合物至关重要.
- 虽然在微生物中很常见,但CAZymes在动物中很少见,除了通过水平基因转移 (HGT) 获得的例外.
- 农业害虫Bemisia tabaci (白) 缺乏经过HGT获得的CAZymes的记录.
研究的目的:
- 在Bemisia tabaci中全面检测HGT获得的CAZymes.
- 描述这些酶的功能和起源.
- 为了比较B. tabaci和相关昆虫之间的CAZyme HGT事件.
主要方法:
- 在B. tabaci.中检测HGT候选物的生物信息分析.
- 遗传学分析以确定酶来源.
- 生物化学测试以确认酶的功能和特异性.
主要成果:
- 在B. tabaci中确定了136个HGT事件,包括14个细菌,真菌和植物起源的CAZymes.
- 在B. tabaci中发现了功能性植物获得的β-葡萄糖酶 (GH17和GH152),这是第一个在动物中表征的.
- 在Trialeurodes vaporariorum和Thysanoptera中发现了较少的HGT获得的CAZymes,这表明了特定的进化事件.
结论:
- 贝米西亚烟草具有独特的植物获得的CAZymes,扩大已知的动物酶谱.
- 来自植物的CAZymes在穿孔吸昆虫的适应中发挥着重要作用.
- 特别是来自植物的CAZymes的HGT是植物食虫的一个关键的进化策略.
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