在双相体外卵细胞成熟系统中添加亲素可调节人类卵细胞和累积细胞转录组
Berta Cava-Cami1, Antonio Galvao2,3,4, Heidi Van Ranst1
1Follicle Biology Laboratory, Research Group Genetics, Reproduction and Development, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
Molecular human reproduction
|January 25, 2025
概括
在体外成熟 (IVM) 介质中补充前素可以通过调节人类IVM周期中的积分细胞和卵细胞转录组来增强卵细胞发育能力. 这可能会改善接受试管婴儿的患者的结果.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 基因组学就是基因组学.
背景情况:
- 双相试管婴儿成熟 (IVM) 为试管婴儿提供了一个患者友好的替代方案,特别是对于高反应性个体.
- 与传统的卵巢刺激相比,目前的IVM协议的累积活产率较低.
- 卵细胞分泌因子 (OSF) 通过影响累积细胞基因表达来增强动物模型中的卵细胞发育能力.
研究的目的:
- 为了研究添加pro-cumulin (GDF9:BMP15异构体) 的人类双相IVM培养对卵细胞和累积细胞的转录组的影响.
- 评估从小毛囊中获取的卵细胞和累积细胞的转录组变化,在最小刺激的,非hCG触发的IVM周期中.
主要方法:
- 人体积卵细胞复合体 (COCs) 在双相IVM系统中培养,有或没有pro-cumulin.
- 单细胞RNA测序 (scRNA-seq) 在卵细胞及其伴随的累积细胞上进行.
- 分析了转录形状,以确定由pro-cumulin诱导的基因表达变化.
主要成果:
- 亲素调高了与累积细胞扩张和增殖相关的基因,同时降低了类固醇生成,化和亡的调节.
- 亲素调节不成熟的卵细胞转录组,影响翻译,亡和线粒体重塑通路.
- 亲素恢复了在采集时部分剥离的成熟的第二阶段转基因卵细胞的转录组概况.
结论:
- 益素调节累积细胞和卵细胞转录体,可能在双相IVM后增加具有发育能力的卵细胞数量.
- 进一步的研究应该评估pro-cumulin的蛋白质效应和对胚胎学结果的影响,特别是对部分剥离的卵细胞.
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