相关实验视频

Updated: May 30, 2025

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection

Published on: October 23, 2009

基于便携式pH测量仪的竞争性免疫检测E-selectin使用尿素酶封装的金属有机框架

Yiyan Xie¹, Xianhuan He¹, Rujie Yu¹

¹Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education of China), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, PR China.

|January 25, 2025

概括

一个新的生物传感器使用酶纳米复合物和便携式pH计准确量化E-selectin (CD62E). 这种方法为检测CD62E提供了一种敏感和选择性的方法,这对于诊断血管疾病至关重要.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
分析化学分析化学

背景情况:

E-选择素 (CD62E) 是内皮细胞上一个关键的粘附分子,在炎症期间升高.
准确量化CD62E对于早期诊断和治疗血管疾病和高血压至关重要.

研究的目的:

开发一种新的,敏感和选择性的方法来定量E-选择素 (CD62E).
使用酶封装的金属有机框架和便携式pH测量仪用于免疫测试和生物传感.

主要方法:

制备两种纳米复合材料:与尿素酶封装的和与CD62E抗体结合的化物伊米达酸框架-8 (U@ZIF-8-Ab) 和CD62E蛋白质涂层的Fe3O4磁珠 (MB-CD62E).
使用一种竞争性抑制试验,其中CD62E干扰了两个纳米复合材料的结合.
在磁性分离后测量尿酶活性导致的pH值变化,以确定CD62E度.

主要成果:

在pH差异和CD62E度的对数 (0.02500 ng mL-1) 之间建立了线性关系.
达到了9.28 pg mL-1的低检测极限.
该方法表现出良好的选择性,可重复性,稳定性,并且需要低剂量的试剂.

结论:

开发的方法提供了一个简单,敏感和选择性的方法来定量E-selectin (CD62E).
这项工作突出了酶封装MOF和便携式pH计在生物传感应用中的潜力.
生物传感器对于早期诊断和监测血管疾病非常有价值.

关键词:

竞争性免疫测试方法电子选择 (E-Selection) 是一个电子选择系统.便携式的pH值计可以携带.尿素的水解是尿素的水解.用尿素封装的MOFs.

更多相关视频

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

相关概念视频

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...