通过Src/JNK信号介导的细胞循环停止和线粒体亡,Corinoline的抗骨髓瘤活性
Mufeng Li1, Jianhang Jiao1, Weibo Jiang1
1Department of Orthopedics, The Second Hospital of Jilin University, Changchun 130041, China.
International immunopharmacology
|January 25, 2025
概括
科林林 (COR) 通过向Src/JNK通路来抑制骨髓瘤 (OS) 细胞生长,并诱导细胞亡. 这种天然化合物对OS治疗有前途,在体内减少瘤大小而无毒性.
科学领域:
- 药理学 药理学是指药理学的学科.
- 在瘤学瘤学.
- 分子生物学分子生物学
背景情况:
- 科里诺林 (COR) 是来自Corydalis bungeana Turcz的类化合物,已知具有治疗性质.
- 它的抗骨髓瘤 (OS) 作用和分子机制在很大程度上是未知的.
- 研究COR在OS治疗中的潜力至关重要.
研究的目的:
- 调查对抗骨髓瘤 (OS) 的COR分子机制.
- 确定COR对OS细胞增殖,细胞亡和细胞循环的影响.
- 确定参与COR反OS活动的关键信号通道.
主要方法:
- 在OS细胞系的体外研究评估了增殖,细胞亡和细胞周期.
- 西方斑分析用于检查蛋白质表达 (Src,JNK,c-JUN,环B1,Ki-67).
- 在体内异种移植的小鼠模型来评估抗瘤疗效和毒性.
主要成果:
- 在剂量取决的方式中,COR抑制了OS细胞的增殖和诱导了亡.
- COR导致G2/M阶段细胞周期停止,并激活Src/JNK通路 (增加p-Src,p-JNK,p-c-JUN).
- 在体内,COR减少了瘤大小而没有毒性,增加了p-JNK和B1环素,同时降低了Ki-67.
结论:
- 通过Src/JNK信号通路,COR有效地抑制骨髓瘤的进展.
- 用COR准Src/JNK通路为骨髓瘤提供了一个潜在的治疗策略.
- 科尔显示出显著的抗瘤活性和OS治疗的有利安全概况.
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