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人类胎盘中与衰老相关的转录的表征

Oren Barak¹, Alexander D Bauer¹, W Tony Parks²

¹Magee-Women's Research Institute, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh, Pittsburgh, PA, 15213, USA.

|January 25, 2025

概括

胎盘中的老化相关分泌表型 (SASP) 转录在晚期怀孕和产科综合征 (如子宫前和胎儿生长限制) 中发生变化. 这些签名可能有助于描述健康和疾病中的胎盘衰老.

科学领域:

产科和妇科产科和妇科
细胞生物学细胞生物学
基因组学就是基因组学.

背景情况:

热囊细胞衰老与产科综合征,如子宫前和胎儿生长限制 (FGR) 有关.
一组特定的28个与衰老相关的分泌表型 (SASP) 转录已在孕前胎盘中确定.

研究的目的:

研究一组定义的胎盘衰老相关分泌表型 (SASP) 转录的调节.
为了确定这些SASP转录是否在晚期胎盘或具有主要产科综合征的怀孕胎盘中受到独特调节.

主要方法:

对来自健康和患病怀孕的胎盘RNA测序数据的分析 (先孕症,FGR,高血压障碍的FGR,早产).
在早产 (<37周) 和晚产 (≥41周) 之间的SASP转录表达的比较.
在低氧,降低分化或亡/亡的条件下,在初级人类热囊细胞中对SASP表达的体外评估.

主要成果:

28个SASP转录中的8个显示出早产和晚产之间存在显著的变化,其中特定的基因上调 (FSTL3,IL1RL1,INHBA,VEGFA) 和其他基因下调 (STC1,RARRES2,MRC2,SELP).
在FGR/高血压障碍怀孕的胎盘中,SASP mRNA表达显著丰富.
在体外研究中,低氧和亡与FSTL3,VEGFA和DKK1.1的改变表达有关.

结论:

一组独特的胎盘SASP标志着晚期胎盘和胎盘功能障碍综合征.
trofhoblastic SASP签名显示出在正常和病态怀孕中表征胎盘衰老的潜力.

关键词:

在FSTL3中使用.胎盘胎盘胎盘胎盘孕前症孕前症在 SASP 找 SASP 衰老是一种衰老.热细胞热细胞

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