相关实验视频

Updated: May 30, 2025

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Published on: May 9, 2016

作为乳腺癌微环境中的祖先特征,原氧化プロ林位点变化的空间定位

Harrison Taylor¹, Laura Spruill², Heather Jensen-Smith³

¹Department of Pharmacology & Immunology, Proteomics Center, Medical University of South Carolina, Charleston, SC, United States.

Matrix biology : journal of the International Society for Matrix Biology

|January 25, 2025

概括

乳腺癌微环境具有独特的原修饰,特别是烯氧化 (HYP),产生生物活性域. 这些HYP位点影响乳腺癌细胞信号传递,并可能影响祖先的乳腺癌特征.

科学领域:

生物化学生物化学
在瘤学瘤学.
蛋白质组学是指蛋白质组学.

背景情况:

乳腺组织微环境中的原-肌体相互作用对乳腺癌风险,进展和结果至关重要.
氨酸化 (HYP) 是一种关键的翻译后修饰,与乳腺癌存活率和进展有关,影响细胞信号和矩阵重塑.

研究的目的:

为了研究乳腺癌微环境是否含有独特的原转化后修饰 (PTMs),形成生物活性域.
关联这些新的原体域与空间病原学特征及其对乳腺上皮细胞信号传递的影响.

主要方法:

在260个不同的乳腺组织样本上使用了质谱成像蛋白质学和综合蛋白质学方法.
分析包括来自癌症基因组图谱 (TCGA) 的瘤切除和三阴性乳腺癌样本.
组织微阵列比较了健康,正常的邻近和侵入性导管癌组织中修改的原体部位周围的蛋白质变化.

主要成果:

在TCGA瘤切除术中观察到增加的prolyl-4-hydroxylase亚单元α-3 (P4HA3) 和纤维状原体的空间调节.
原α-1 ((I) 链上的特定域位点在三阴性乳腺癌中显示出空间调节.
在正常的邻近和侵袭性乳腺癌组织之间,与特定位点的氨酸氧化相关的原蛋白α-1(I) 链域 (aa 506-514) 发生了显著的变化.

结论:

局部化原HYP在乳腺癌微环境中形成新的生物活性域.
这些域分布在空间上,并影响乳腺癌上皮细胞的增殖,化学反应和信号传递.
这些发现可能有助于理解乳腺癌的祖先特征.

关键词:

他们的祖先的祖先.生物标志物生物标志物乳腺癌乳腺癌乳腺癌原蛋白是一种原蛋白.细胞外矩阵是细胞外矩阵.氧化普罗林是一种氧化普罗林.图像质谱仪的成像质谱仪类成像成像类成像酸是一种酸.种族种族种族种族斯特罗马斯特罗马斯特罗马斯特罗马电流模式电流模式

更多相关视频

A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment

A Label-Free Segmentation Approach for Intravital Imaging of Mammary Tumor Microenvironment

Published on: May 24, 2022

Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools

Quantifying Fibrillar Collagen Organization with Curvelet Transform-Based Tools

Published on: November 11, 2020

相关概念视频

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

Structural Protein Function

Structural Protein Function

Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...