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重组性人参与氨基酸并列重复的表达,特征和抗结肠癌活性

Yu Feng¹, Weina Li¹, Weigang Yuwen²

¹Engineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, School of Chemical Engineering, Northwest University, Xi'an, 710069, China; Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an, 710069, China; Biotech. & Biomed. Research Institute, Northwest University, Xi'an, 710069, China.

Protein expression and purification

|January 25, 2025

概括

重组性人参,AT0601和AT0605的表达成功. AT0605对结肠癌细胞的抗癌活性明显高于AT0601,提供了一个更强大的治疗候选者.

科学领域:

生物技术是生物技术.
药理学药理学是指药理学的学科.
分子生物学分子生物学

背景情况:

人参酸是有价值的生物活性化合物,但它们的天然提取和化学合成是昂贵和具有挑战性的.
有限的研究存在于大规模生产和特定的人参的治疗潜力.

研究的目的:

为了在 Bacillus subtilis WB600.00 中表达复合性人参,AT0601 和 AT0605.
评估和比较AT0601和AT0605对结肠癌CT26细胞的抗癌活性.
阐明这些复合性人参的潜在作用机制.

主要方法:

人参六FKEHGY (AT0601) 的重组表达及其合重复AT0605 在细菌亚细菌 WB600.00.
在体外生物活性测试以确定对CT26结肠癌细胞的半最大抑制度 (IC50).
细胞周期分析 (G1阶段停止) 和亡诱导研究,包括酶通路激活和线粒体膜潜能评估.

主要成果:

再组合AT0601和AT0605.5的成功表达
与AT0601相比,AT0605对CT26细胞的抗癌活性明显高,IC50低50倍 (16.82 ± 1.3μM与855.57 ± 6.04μM在48小时).
这两种都诱导了G1阶段细胞周期停止,激活了与线粒体相关的酶通路,并降低了线粒体膜潜能,导致了亡.

结论:

再组合表达为生产人参酸提供了一种可行的方法.
AT0605是一种高效的抗癌药物,可以对抗结肠癌CT26细胞,超过AT0601.
这些的抗癌作用是由细胞循环停止和通过线粒体通路诱导细胞亡的介导.

关键词:

这种细菌是 Bacillus subtilis.串联序列重复的重复这是一种抗结肠癌的药物.复合性人参酸是人参的复合性人参.

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