Cry2Aa蛋白质不足以构成对Pardosa astrigera的威胁
Weijiao Liu1, Qiqing Yu1, Muhammad Nasir2
1Zhengzhou Research Base, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China; National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji 831100, China.
International journal of biological macromolecules
|January 25, 2025
概括
转基因Bacillus thuringiensis (Bt) 的作物对蜘蛛肠细菌产生影响. 在Pardosa astrigera蜘蛛中,Cry2Aa蛋白改变了微生物群落和新陈代谢,观察到性别特异性的影响.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 毒理学 毒理学 毒理学
背景情况:
- 转基因作物表达Bacillus thuringiensis (Bt) 毒素的转基因作物被广泛商业化.
- 评估Bt毒素对非目标生物体 (如捕食者Pardosa astrigera) 的影响至关重要.
- 由于活动和营养需求不同,P. astrigera对Bt毒素的性别特异反应是可能的.
研究的目的:
- 研究Cry2Aa蛋白暴露对P. astrigera生存,发育和共生细菌群落的影响.
- 为了确定性别是否影响P. astrigera对Cry2Aa蛋白的反应.
- 分析与Cry2Aa暴露相关的微生物新陈代谢的功能变化.
主要方法:
- 对P. astrigera暴露于含有Cry2Aa蛋白质的猎物.
- 监测生长和发育期间的生存率和体重.
- 16S rRNA测序以分析肠道微生物社区的组成和多样性.
- 代谢途径的功能丰富分析.
主要成果:
- Cry2Aa蛋白没有影响P. astrigera的存活率或体重.
- 观察到肠道共生细菌多样性和组成的显著变化.
- 在Cry2Aa暴露后,占主导地位的细菌类从Actinobacteria/Corynebacterium-1转移到Firmicutes.
- 性别特定的变化包括雌性菌的丰富性增加和改变的Bacillus和Weissella水平.
结论:
- 暴露于Cry2Aa蛋白质显著改变了P. astrigera的肠道微生物群,以一种取决于性别的方式.
- 功能分析揭示了碳水化合物和核酸代谢等代谢途径的性别特异性变化.
- 这些发现支持不同的蜘蛛策略来应对Bt毒素,并为转基因作物的环境安全评估提供信息.
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