相关实验视频
Updated: May 5, 2026

05:05
A Modified Precipitation Method to Isolate Urinary Exosomes
Published on: January 16, 2015
17.5K
尿蛋白学定义了一个与免疫检查点相关的炎特征
James P Long1, Shailbala Singh2, Yanlan Dong3
1Department of Biostatistics, Division of Discovery Science, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
Journal for immunotherapy of cancer
|January 25, 2025
概括
一种新的非侵入性尿检可以准确检测由免疫检查点抑制剂 (ICI) 引起的急性间歇性炎 (AIN),避免侵入性活检. 这种生物标志物签名有助于早期治疗,并保持癌症治疗的有效性.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學.
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
背景情况:
- 免疫检查点抑制剂 (ICI) 治疗对于癌症治疗至关重要,但可能导致严重的损伤,特别是急性间歇性炎 (AIN).
- 诊断ICI-AIN目前依赖于侵入性脏活检,导致治疗延迟和潜在的伤害.
- 非常需要非侵入性生物标志物来准确区分ICI-AIN与急性损伤 (AKI) 的其他原因.
研究的目的:
- 开发和验证ICI-AIN使用高灵敏度蛋白质组学的非侵入性诊断策略.
- 确定新的尿液和血生物标志物,以区分ICI-AIN与非ICI-AIN导致AKI的原因.
- 通过早期诊断和ICI-AIN的有针对性的治疗来改善患者的治疗结果.
主要方法:
- 利用NUcleic acid链接免疫三明治测试 (NULISA) 技术,精确量化尿液和血样本中的200多种炎症蛋白.
- 分析了接受ICI治疗的AKI患者的样本.
- 采用统计和机器学习方法来构建诊断生物标记符号.
主要成果:
- 尿样显示出比血更高的敏感度和特异性,用于诊断ICI-AIN.
- 确定了新的尿液生物标志物,包括IL-5,Fas,以及已确认的标志物,如CXCL9和TNF-α.
- 开发了一种尿液生物标记特征 (IL-5+Fas),在ICI-AIN诊断中达到0.94的AUC.
结论:
- 高灵敏度蛋白质组学为诊断ICI-AIN提供了一种可行的非侵入性方法.
- 这种诊断策略可以促进及时干预,减轻ICI引起的损伤,并优化癌症治疗.
- 允许在有ICI-AIN病史的患者中安全地重新进行ICI治疗.
相关概念视频
Nephrotic Syndrome I : Introduction
1.2K
Nephrotic Syndrome is a chronic kidney disorder defined by clinical findings such as severe proteinuria, hypoalbuminemia, hyperlipidemia, and edema. These symptoms result from damage to the glomeruli, the kidney’s filtering units, increasing their permeability to proteins.Definition and Meaning:Proteinuria, defined as the loss of more than 3.5 grams of protein per day in adults, is a crucial feature of nephrotic syndrome. This condition is often accompanied by edema, the accumulation of...
1.2K
Nephrotic Syndrome II : Assessment and Medical Management
409
IntroductionNephrotic syndrome is a kidney disorder marked by excessive protein loss in the urine, leading to various systemic complications. This condition often results from damage to the glomeruli—the kidney's filtering units—causing proteinuria, low blood protein levels, and fluid retention. Understanding the assessment, diagnosis, and management of nephrotic syndrome is essential for effective treatment and prevention of further kidney damage.AssessmentPatient History: Document...
409
Urine Studies I: Urinalysis
2.6K
Urinalysis is a widely used diagnostic test that analyzes urine's physical, chemical, and microscopic characteristics. Healthcare providers use it to detect and monitor various health conditions, including renal disease, urinary tract infections (UTIs), diabetes, and metabolic or systemic disorders.Components of UrinalysisUrinalysis consists of three primary components: physical, chemical, and microscopic examination. Each provides unique insights into the urine sample and, by extension, the...
2.6K
Acute Kidney Injury IV: Diagnostic Studies and Prevention
559
Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
559
Diabetic Nephropathy
32
Definition Diabetic nephropathy is a chronic kidney complication that results from prolonged hyperglycemia.Prevalence It is the most common cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD) worldwide, affecting up to half of individuals with diabetes.Pathophysiology • Sustained hyperglycemia triggers multiple hemodynamic and metabolic changes in the kidney. • Early in the disease, increased renal blood flow and glomerular hyperfiltration...
32

