生物素a通过TAS2Rs激活调节人体颗粒细胞中的类固醇生成和细胞代谢:卵巢功能的焦点
Francesca Paola Luongo1, Sofia Passaponti2, Alesandro Haxhiu3
1Department of Molecular and Developmental Medicine, Siena University, Siena, 53100, Italy. Francesca.luongo@unisi.it.
生物素A (BCA) 是一种内分泌干扰化学物质,激活了人体颗粒细胞中的苦味受体 (TAS2Rs). 这种激活会影响基因表达和细胞代谢,可能会影响生殖健康.
科学领域:
- 内分泌学 在内分泌学.
- 细胞生物学 细胞生物学
- 毒理学 毒理学 毒理学
背景情况:
- 内分泌干扰化学物质 (EDC) 破坏激素系统,影响生殖健康.
- 生物素A (BCA),一种异黄,表现出类似雌激素的特性,被确定为EDC.
- 主要人体颗粒细胞 (GCs) 对于卵巢功能和激素产生至关重要.
研究的目的:
- 研究BCA对人类GCs转录,新陈代谢和激素调节的影响.
- 确定苦味受体 (TAS2Rs) 在调解BCA的作用中的作用.
- 阐明BCA对参与类固醇生成和细胞能量动态的关键基因的影响.
主要方法:
- 主要的人类GC被用BCA (10μM) 治疗.
- 使用选择性抗剂阻止了TAS2R激活.
- 分析了基因表达 (TAS2R14,TAS2R43,StAR,CYP17A1),细胞内,脂滴大小和线粒体网络复杂性.
主要成果:
- BCA上调调节了TAS2R14和TAS2R43,增加了STAR和CYP17A1mRNA水平.
- TAS2R对抗剂逆转了基因表达中的BCA诱导的变化.
- BCA改变了细胞内,脂质滴滴大小,增强了线粒体网络的复杂性,效果被对抗剂逆转.
结论:
- BCA通过TAS2R激活调节GC中的转录和代谢过程.
- TAS2Rs在颗粒细胞内的内分泌调节中起着重要作用.
- 进一步研究EDC对生殖健康的影响,就像BCA的影响一样,对于保持生育是至关重要的.
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