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精子DNA碎片化指数:在辅助生殖技术治疗中对预测胚胎质量的有效性有限

Huan Jiang¹, Xiaolu Xia¹, Ying Luo²

¹Department of Reproductive Endocrinology, Longgang District Maternity & Child Healthcare Hospital of Shenzhen City (Longgang Maternity and Child Institute of Shantou University Medical College), Shenzhen, China.

Reproductive biology and endocrinology : RB&E

|January 25, 2025

概括

精子DNA碎片化指数 (DFI) 影响辅助生殖技术 (ART) 中的受精率,但对其他胚胎发育的预测能力有限. 预测受精成功的DFI截止值为21.15%被确定.

科学领域:

生殖医学生殖医学
安德罗学与人类学
胚胎学胚胎学

背景情况:

男性不孕症诊断和ART结果预测的精子DNA碎片化指数 (DFI) 门缺乏标准化,在15-30%之间变化.
评估DFI对早期胚胎发育的影响对于完善ART协议至关重要.

研究的目的:

评估精子DFI在ART期间对早期胚胎发育的影响.
为了确定可靠的DFI截止值来预测ART成功.

主要方法:

从2021-2022年对913个ART周期 (1,476个试管婴儿,295个ICSI) 的回顾性分析.
精液分析遵循了世卫组织的指导方针;DFI通过阿克里丁色试验 (AOT) 确定.
DFI,男性/女性因素和胚胎学参数之间的相关性分析;截止值的ROC分析.

主要成果:

精子DFI与男性年龄正相关,与精子运动性,度和形态学负相关.
在孤立的IVF/ICSI组中,没有发现DFI与其他胚胎学参数之间的显著关联.
在所有ART周期中,DFI和受精率之间观察到负相关性;ROC分析表明,预测高受精率的DFI截止值为21.15%.

结论:

精子DFI与受精率有负相关性,但对其他胚胎学结果具有有限的预测效果.
DFI评估可以帮助预测早期胚胎发育,特别是在老年男性或精子质量差的男性中.
21.15%的DFI值可能有助于预测ART期间的受精率.

关键词:

辅助生殖技术辅助生殖技术施肥率是指受精率是指受精率.在试管婴儿受精体外受精.在细胞体内注射精子.精子DNA碎片化指数

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