相关实验视频

Updated: May 30, 2025

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Published on: June 16, 2019

从多能干细胞生成HLF+HOXA+人类造血原生细胞的协议

Sherry Li Zheng¹, Jonas L Fowler¹, Julie Y Chen¹

¹Institute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA.

|January 26, 2025

概括

这项研究详细介绍了从人类多能干细胞 (hPSC) 制造血造干细胞 (HSC) 的10天方案. 这种方法在单层培养中产生血液和免疫原生细胞,用于研究应用.

科学领域:

干细胞生物学干细胞生物学
血液形成血液形成血液形成
发育生物学是发展生物学.

背景情况:

造血干细胞 (HSC) 对于生成所有血液和免疫细胞至关重要.
了解HSC的发展是再生医学和疾病建模的关键.
现有的在体外生成HSC的方法可能是复杂和耗时的.

研究的目的:

提出一种新的,高效的协议,用于将人类多能干细胞 (hPSCs) 分化为造血原体.
为了使表达关键HSC转录因子 (HLF,HOXA5,HOXA7,HOXA9,HOXA10) 的细胞产生.
建立一种可扩展的方法,在单层培养中生产血液和免疫细胞.

主要方法:

人类多能干细胞 (hPSCs) 被解离并播种.
序列分化涉及定义的,无血清介质的添加.
该协议引导细胞通过后原始条纹,侧面半皮,动脉内皮和血源内皮阶段.
最后阶段产生了造血原始体.

主要成果:

这项为期10天的协议成功地从hPSCs中产生了造血原始体.
这些原始体表达了基本的HSC转录因子:HLF,HOXA5,HOXA7,HOXA9和HOXA10.
该方法适用于单层培养,方便制造.

结论:

该协议提供了一种简化的方法,可以从hPSCs中生成HSC类细胞.
生成的造血原体对研究血液和免疫细胞发育有价值.
这种方法支持大规模制造血液和免疫细胞的潜力.

关键词:

细胞分化的细胞分化.发育生物学是发展生物学.干细胞是干细胞的组成部分.

更多相关视频

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Published on: November 1, 2017

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Published on: December 3, 2015

相关概念视频

Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...