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基于金属有机框架的调节平台,用于在非水性系统中增强活性的脂酶的固定

Hongde An¹, Nanxin Gong¹, Hao Chen¹

¹College of Life Science, Hebei University, Innovation Center for Bioengineering and Biotechnology of Hebei Province, Baoding 071002, China.

International journal of biological macromolecules

|January 26, 2025

概括

金属有机框架 (MOF) 为酶固定提供了一个可调的平台. 混合脂酶@NKMOF-101生物催化剂表现出增强的活性和稳定性,金属离子提高了高级酶@MOF应用的性能.

科学领域:

材料科学材料科学材料科学
生物催化剂是一种生物催化剂.
纳米技术纳米技术

背景情况:

金属有机框架 (MOF) 越来越多地用于酶固定,因为它们的多孔结构和化学多功能性.
酶固定增强了酶的稳定性,可重复使用性和各种应用中的催化效率.

研究的目的:

使用易于在现场封装方法构建和验证混合酶@MOF生物催化剂.
研究MOF特性 (粒子大小,金属离子,酶分布) 对固定脂酶活性和选择性的影响.
评估开发的酶@MOF生物催化剂的稳定性和可重复使用性.

主要方法:

在NKMOF-101结构中的脂酶的in situ封装,其中包含各种金属离子 (Mg,Mn,Zn,Co,Ni).
在MOF中对脂酶的封装和固定的特征.
对催化活性,基质亲和力 (K_m) 和自由和固定脂酶的选择性进行测试.
研究MOF粒子大小,金属离子和酶分布对生物催化剂性能的影响.
可回收性和长期储存稳定性测试.

主要成果:

与脂酶@ZIF-8和脂酶@MCM-41相比,脂酶@NKMOF-101-Mn的催化活性显著提高.
lipase@NKMOF-101s 的降低K_m值表明非水性系统中的基质亲和力增强.
减少MOF颗粒大小导致明显的酶活性急剧增加.
在MOF中,金属离子表现出激活效应,而脂酶@NKMOF-101-Mn显示出转化化的活性增加了约12倍,而不是自由脂酶.
由于特定的酶分布,脂酶@NKMOF-101-Co和-Ni显示了基质选择性.
在5次回收和30天的储存后,Enzyme@MOFs保留了80%以上的活性.

结论:

NKMOF-101作为一个可调和和可持续的平台,用于创建高性能酶@MOF生物催化剂.
开发的生物催化剂为酶应用提供了卓越的催化活性,亲和力,选择性和稳定性.
定制MOF属性可以优化酶固定化,从而提高生物催化剂的性能.

关键词:

增强了活动的活力.利帕斯 (Lipase) 是一种脂肪酶.金属有机框架的框架.非水性系统的非水性系统一个合成合成.

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