转录组分析揭示了两种体外模型的宿主-三动物相互作用模型之间的显著差异
Ekaterina A Lishai1, Dmitry V Ponomarev2, Oxana G Zaparina2
1Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG SB RAS), 10 Akad. Lavrentiev Ave., Novosibirsk, 630090, Russia; Department of Natural Sciences, Novosibirsk State University, 2 Pirogova Str., Novosibirsk, 630090, Russia.
Acta tropica
|January 26, 2025
概括
选择正确的细胞模型对于研究肝炎感染至关重要. 这项研究发现,人类细胞对活虫和寄生虫产品的反应方式存在显著差异,影响基因表达和细胞因子释放.
科学领域:
- 寄生虫学的寄生虫学
- 分子病原体的产生.
- 细胞生物学 细胞生物学
背景情况:
- 菌酶的研究需要有效的体外模型来研究寄生虫感染.
- 猫 (Opisthorchis felineus) 是一种通过食物传播的三角动物,在人类中引起慢性炎症和癌前胆道病变.
- 为复杂的三动物生命周期选择合适的体外模型仍然是一个挑战.
研究的目的:
- 评估两种体外模型,用于研究人类胆管细胞 (H69细胞) 中的Opisthorchis felineus感染.
- 为了比较H69细胞对与活虫共同培养的反应与寄生虫衍生的分泌分泌产物 (ESP).
- 为了确定两个模型之间的分子致病学差异.
主要方法:
- 与活生生的猫虫Opisthorchis猫虫一起培养H69细胞.
- 用寄生虫衍生的分泌分泌产物 (ESP) 化H69细胞.
- 评估细胞增殖,迁移,细胞周期,细胞因子生产和全基因组转录组分析.
主要成果:
- 在活虫共养和ESP暴露模型之间观察到显著的差异.
- 在IL-6,IL-4和TNF细胞因子产生的实质性差异.
- 差异性基因表达:有144个活虫基因与ESP的537个基因相比;只有17个常见的差异性表达基因.
- 功能丰富分析揭示了每个模型的不同受影响的信号通路.
结论:
- 实验室模型的选择显著影响观察到的宿主-寄生虫相互作用和分子反应.
- 暴露于ESP会引起比H69细胞中活虫共同培养更广泛的转录基因反应.
- 这些发现强调了在虫病研究中需要仔细选择模型,并突出了胆道瘤所涉及的途径.
- 需要进一步调查与肝感染相关的癌前胆道病变.
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