在一个针对脱氧尼瓦伦的DNA Aptamer 中解读联结位点
Ruiting Sun1, Rugiya Alieva2, Sergey Belousov3
1Northeast Normal University, Changchun, 130000, China.
Biochemistry. Biokhimiia
|January 26, 2025
概括
这项研究确定了用于检测脱氧尼瓦伦醇的DNA合体的结合位结构. 这种体被用于传感器,以快速检测小麦中的真菌毒素,确保食品安全.
科学领域:
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
- 分析化学 分析化学
背景情况:
- 菌毒素是有毒的真菌代谢物,由于它们在加工过程中的稳定性,对食品安全构成重大威胁.
- 目前在作物中的真菌毒素检测方法往往不够快速或简单,不足以进行早期检测.
- 基于aptamer的传感器为敏感和特定的真菌毒素检测提供了一个有希望的解决方案.
研究的目的:
- 解读一种针对脱氧尼瓦伦醇 (DON) 开发的DNA合体的结合位结构.
- 为了在新型传感器中利用特征化的aptamer,在粮食作物中快速检测真菌毒素.
主要方法:
- 一个针对deoxynivalenol的DNA吸收体的结构分析.
- 开发基于表面增强拉曼光谱 (SERS) 的传感器.
- 在SERS传感器中集成最短,高亲和度的aptamer作为识别元件.
- 在小麦样本中检测出脱氧尼瓦伦醇.
主要成果:
- 对脱氧尼瓦伦醇的DNA合体的结合部位被阐明,揭示了特定的结构特征.
- 确定了对脱氧尼瓦伦醇具有较高亲和力的最短的体.
- 在SERS基于的传感器中成功使用了aptamer来检测小麦中的脱氧尼瓦伦醇.
结论:
- 这种特征的DNA体为deoxynivalenol提供了一个特定的识别元素.
- 开发的基于SERS的食欲传感器可以快速简单地检测作物中的真菌毒素.
- 这种方法有助于提高食品安全,允许早期检测真菌污染.
相关概念视频
Ligand Binding and Linkage
4.7K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.7K
Conserved Binding Sites
4.2K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.2K
Ligand Binding Sites
12.7K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
12.7K
Allosteric Proteins-ATCase
5.7K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.7K
Labeling DNA Probes
8.1K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.1K


