相关实验视频

Updated: Jun 13, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

韩国草 (Eleusine indica) 的染色体规模基因组组合

Solji Lee¹, Changsoo Kim^2,3

¹Department of Crop Science, Chungnam National University, Daejeon, 34134, Republic of Korea. solji2m@o.cnu.ac.kr.

Scientific data

|January 26, 2025

概括

研究人员开发了一种来自韩国的侵入性草 (Eleusine indica) 的高质量基因组组. 本资源有助于了解杂草遗传学,并改进对这种入侵物种的作物管理策略.

科学领域:

植物科学植物科学
基因组学就是基因组学.
杂草生物学杂草生物学

背景情况:

草 (Eleusine indica) 是一种侵入性杂草,与作物竞争资源.
现有的基因组组不完全代表野生草种群的遗传多样性.

研究的目的:

为韩国的一种Eleusine indica.种群构建染色体级的基因组组件.
为了解和管理入侵性草提供一个有价值的遗传资源.

主要方法:

使用PacBio长读和Illumina短读技术进行全基因组测序.
使用Pore-C测序将连接物在伪染色体上.
通过BUSCO分析评估基因组完整性.

主要成果:

产生了Eleusine indica的高质量的染色体级基因组组合.
该组件通过BUSCO分析实现了97%的完整性.
基因组包含97%的重复序列和26836个预测的蛋白质编码基因.

结论:

新的基因组组合代表了一个地理上独特的Eleusine indica.种群.
这种资源将促进草遗传学和杂草控制策略的研究.

更多相关视频

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

Published on: November 1, 2014

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Lampbrush Chromosomes

Lampbrush Chromosomes

In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...