在抗生素选择性压力下的废水系统中,对抗生素耐药性基因和移动遗传元素的metatranscriptomic时间序列洞察力
An-Dong Li1,2,3, Yuanfang Chen1,3, Lei Han2,4
1Engineering Research Center of Health Emergency, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
BMC microbiology
|January 26, 2025
概括
污水安培暴露增加了特定的抗生素耐药性基因 (P14171) 和移动基因元素,表明潜在的健康风险,并加速了活性污泥中的水平基因转移. 这项研究强调了环境系统中抗性基因的动态转录反应.
科学领域:
- 环境微生物学环境微生物学
- 污水处理 污水处理 污水处理
- 抗生素耐药性 抗生素耐药性
背景情况:
- 废水系统作为抗生素耐药性基因 (ARG) 和移动基因元素 (MGE) 的关键枢纽,连接人类和环境.
- 抗生素的使用是这些系统内ARG和MGE扩散的已知驱动因素.
- 了解ARG和MGE对抗生素压力的反应的动态转录反应至关重要.
研究的目的:
- 在暴露于安培的活性污泥中研究ARG和MGE的转录组概况.
- 为了确定安皮西林度对ARG和MGE转录的影响.
- 评估在抗生素压力下水平基因转移 (HGT) 的潜力.
主要方法:
- 批量实验是使用活性污泥进行的,该污泥剂量为安培林,最终度为100和20毫克/升.
- 使用UPLC-MS/MS.来量化西林度.
- 在10个小时的时间序列中进行了metatranscriptomic测序,以对相关数据库进行ARG和MGE转录档案的分析.
主要成果:
- 皮素被快速生物降解,在4到8.5小时内观察到完全去除.
- 虽然DNA水平的ARG配置文件保持稳定,但观察到A类β-乳酸酶 (P14171基因) 的显著转录上调.
- 皮素刺激了MGE,特别是TnpA和sul1基因的转录,但在转录水平上没有发现ARG和MGE之间的显著相关性.
结论:
- 鉴定出P14171基因是唯一对活性污泥中安培素降解有显著反应的ARG,突出了其潜在的健康风险.
- 外部安皮西林暴露可以通过增加MGE转录来促进废水系统中的水平基因转移 (HGT).
- 该研究强调了监测ARG和MGE的转录变化的重要性,以了解环境环境中的抗生素耐药性动态.
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