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脂聚糖类会导致获得的CFTR功能障碍在Murine鼻腔航空公司

Jessica W Grayson¹, T Graham Norwood¹, Shaoyan Zhang¹

¹Department of Otolaryngology-Head and Neck Surgery, University of Alabama at Birmingham Heersink School of Medicine, Birmingham, Alabama, USA.

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery

|January 26, 2025

概括

在小鼠中,细菌脂聚糖 (LPS) 暴露导致CFTR功能的缺陷,影响鼻气道中的化物运输. 该模型有助于研究慢性鼻炎和潜在的CFTR疗法.

科学领域:

呼吸系统生理学呼吸系统生理学
分子生物学分子生物学
病理学病理学病理学

背景情况:

囊性纤维化 (CF) 涉及化物 (Cl-) 运输受损,影响粘膜细胞清除 (MCC).
获得的CFTR功能障碍是非CF个体慢性鼻炎 (CRS) 的潜在因素.
以前的研究表明,细菌脂多糖 (LPS) 可以诱导CFTR功能障碍.

研究的目的:

为了确定小鼠鼻气道中的LPS暴露是否可以创建获得CFTR功能障碍的模型.
为了研究LPS对化物运输和鼻腔组织病理学的影响.

主要方法:

在CFTR+/+小鼠鼻腔气道中,每天两次使用LPS或载体治疗,持续一周.
通过鼻潜在差异 (NPD) 试验评估了横体化物运输.
组织病理学分析评估了淋巴结合物和上皮/亚上皮高度.

主要成果:

暴露于LPS显著降低了在小鼠鼻腔气道中的化物分泌.
阿米洛里德敏感电压没有受到影响,这表明特定的CFTR介导的传输损伤.
组织病理学显示,在接受LPS治疗的小鼠中,淋巴结合物增加和上皮质加厚.

结论:

在小鼠鼻腔气道中LPS灌会导致体内化物运输中获得的缺陷.
这种小鼠模型适用于研究CRS中获得的CFTR功能障碍.
该模型可用于测试CFTR调节器以改善MCC.

关键词:

在CFTR中,CFTR是最重要的.慢性犀牛鼻炎慢性鼻炎慢性鼻炎慢性鼻炎组织病理学组织病理学脂多糖类糖化物 (Lipopolysaccharide) 是一种脂多糖类糖.鼻腔潜能差异的不同鼻炎是一种鼻炎.穿越性皮质离子运输

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