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使用NIR-II光纳米探针对肠道细菌进行图像成像,轻松标记阴性细菌

Wenwen Chen¹, Rong Chen¹, Yutong Sun¹

¹College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, P. R. China.

|January 27, 2025

概括

这项研究引入了一种新的近红外II (NIR-II) 光纳米探针策略,用于实时跟踪小鼠的肠道细菌分布. 该方法使用硫化银量子点来可视化胃肠道中的阴性细菌.

科学领域:

生物医学工程生物医学工程
微生物学微生物学
纳米技术纳米技术

背景情况:

肠道细菌对健康和疾病至关重要,需要准确的体内分布跟踪来进行诊断.
目前的非侵入性成像方法缺乏肠道细菌实时定位能力.
了解细菌生物分布是解读生理和病理作用的关键.

研究的目的:

开发一种非侵入性成像策略,用于实时跟踪肠道细菌的生物分布.
使用近红外II (NIR-II) 光纳米探测器创建一种新的可视化技术.
通过细菌局部化,使肠道相关疾病的早期诊断和理解成为可能.

主要方法:

开发了一种使用正电荷的硫化银量子点 (Ag2S QDs) 作为NIR-II纳米探针的标签策略.
利用静电相互作用来标记负电荷的グラム阴性细菌,特别是*Escherichia coli*Nissle 1917.
在小鼠胃肠道中使用NIR-II光成像用于高时空分辨率可视化.

主要成果:

通过Ag2S QDs实现了格兰阴性细菌的快速有效标记.
实时实体追踪鼠标胃肠道中标记的益生菌.
使用NIR-II成像,获得了高时空分辨率的细菌分布可视化.

结论:

使用NIR-II光纳米探针为肠道细菌建立了一个强大的成像策略.
介绍了用于生物医学应用的纳米材料-细菌混合系统的新概念.
开发的技术有助于实时体内追踪肠道细菌,帮助疾病诊断和研究.

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