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在MdNAC5中的自然变异有助于果实坚硬性和果成熟的分歧

Li Liu^1,2, Yuanji Wang¹, Jianhua Guo¹

¹State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China.

Horticulture research

|January 27, 2025

概括

研究人员发现了一种基因变异,MdNAC5,它影响果的坚硬性和成熟. 操纵这种基因为果培育提供了一种新的策略,使果具有所需的坚固性和成熟度特征.

科学领域:

植物遗传学植物遗传学
果实科学是一门学科.
分子生物学分子生物学

背景情况:

果实坚硬度是果质量,成熟度和价值的关键指标.
果实坚硬是一种复杂的特征,受多个基因的影响.
全基因组重新排序是研究复杂特征的强大工具.

研究的目的:

为了确定调节果实坚硬性和果成熟的遗传因素.
了解导致水果坚硬度变化的分子机制.

主要方法:

在294种果杂交品种的全基因组重新测序.
高密度遗传地图的构建.
定量特征位置 (QTL) 映射.
基因表达分析和MdNAC5.5的功能验证.

主要成果:

用5014个bin标记器构建了一个高密度的binmap.
确定了MdNAC5中的A-T等位基因变异,作为水果坚硬性和成熟的潜在调节者.
过度表达MdNAC5变体改变了甲和1-氨基cyclopropanecarboxylic 酸的产生,影响了度和成熟.
MdNAC5变种与MdACS1和MdERF3的促进子区域的差异性结合影响了基因表达.

结论:

MdNAC5基因及其等位基因在调节果坚硬度和成熟方面发挥着重要作用.
了解MdNAC5功能为果育种计划中操纵水果坚度提供了一种策略.
准MdNAC5为开发具有更好的质量和储存特征的果提供了一条途径.

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