相关实验视频

Updated: May 30, 2025

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Using Phylogenetic Analysis to Investigate Eukaryotic Gene Origin

Published on: August 14, 2018

在Erica (Ericaceae) 中最大限度地提高基于捕获目标的基因组学信息性

Seth D Musker^1,2, Nicolai M Nürk², Michael D Pirie³

¹Department of Biological Sciences, University of Cape Town, Rondebosch, Cape Town, South Africa University of Bayreuth Bayreuth Germany.

|January 27, 2025

概括

开发一套定制的植物遗传学目标,特别是对于Erica属,显著提高了遗传学分辨率. 这种方法增强了数据的信息性和内部恢复,克服了复杂进化研究中通用基因组的局限性.

科学领域:

植物遗传学和植物遗传学.
获取和分析基因组数据.

背景情况:

目标捕获测序 (Hyb-Seq) 是植物基因组学的标准方法,可以研究许多基因组区域 (位置).
一个主要的挑战是选择合适的基因位点,特别是对于缺乏先前存在的基因组资源的非模型生物.
现有的通用基因组可能缺乏足够的植物遗传信号来快速辐射或复杂的植物群.

研究的目的:

为了设计一个定制的目标捕获设置为埃里卡的基因组学,以最大限度地提高基因组学信息性,并最大限度地减少对比.
通过将广泛使用的Angiosperms353集的基因纳入目标组的普遍性.
在基因恢复,信息内容和内部恢复方面评估定制目标的性能.

主要方法:

为Erica设计了一套300多个基因的定制目标组,包括来自Angiosperms353组的保存基因.
应用了约90种Erica物种和相关外组 (Calluna,Daboecia,Rhododendron) 的目标捕获测序.
分析了恢复率,节信息网站和四极管内部解像度概率 (QIRP) 的数据,将QIRP与内子含量相关联.

主要成果:

在不同的Erica物种和外组中实现了出色的目标恢复率.
证明了高的遗传学信息含量,QIRP与内子含量正相关.
发现直接将内流体纳入目标集,与外流体附带捕捞相比,内流体的回收得到了大幅度的改善.

结论:

构建定制目标集是非常有价值的,以克服植物遗传学中普遍集的局限性.
开发的埃里卡特异性目标集有效地最大限度地提高了植物遗传信息性和内部恢复.
提供了开源工具,以促进这种方法在其他植物群体中复制.

关键词:

生物信息学是一种生物信息学.这是Ericaceae.人类基因组学是什么?目标捕获目标捕获

更多相关视频

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

相关概念视频

Gene Evolution - Fast or Slow?

Gene Evolution - Fast or Slow?

The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Phylogenetic Trees

Phylogenetic Trees

Phylogenetic trees come in many forms. It matters in which sequence the organisms are arranged from the bottom to the top of the tree, but the branches can rotate at their nodes without altering the information. The lines connecting individual nodes can be straight, angled, or even curved.