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相关概念视频

High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

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High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

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In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
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High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
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Gas Chromatography: Types of Columns and Stationary Phases01:17

Gas Chromatography: Types of Columns and Stationary Phases

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Gas chromatography (GC) relies on stationary phases to separate and analyze components in a sample. There are two main types of stationary phases: liquid and solid. Liquid stationary phases are non-volatile, thermally stable, and chemically inert liquids coated onto the column. Solid stationary phases are particles of adsorbent material, such as silica gel or molecular sieves.
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
470
Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

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Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
182
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants
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开发一种基于正压的仪器仪表,用于高效的固相提取.

Pawankumar Rai1, Srishti Mehrotra1,2, Vaibhavi Lahane3,2

  • 1Food Toxicology Group, CSIR-Indian Institute of Toxicology Research, Vishvigyan Bhawan, 31, Mahatma Gandhi Marg, Lucknow, Uttar Pradesh 226001 India.

Journal of food science and technology
|January 27, 2025
PubMed
概括

开发了一种新的固相提取 (SPE) 方法和组装,以克服食品样品制备方面的局限性. 这种技术可以防止化合物蒸发,改善汁中挥发性和热敏化合物的回收和分析.

关键词:
提取 提取 提取果汁是一种果汁.这是LC-HRMS.破坏标记的标记

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科学领域:

  • 食品科学 食品科学 食品科学
  • 分析化学 分析化学
  • 样品的准备 样品的准备

背景情况:

  • 固相提取 (SPE) 对于食品矩阵分析至关重要,但其回收率低,可复制性差,挥发性化合物损失.
  • 传统的SPE方法通常需要蒸发步骤,从而有可能丢失挥发性和热敏分析剂.

研究的目的:

  • 开发一种创新的SPE方法和提取组件,以更好地从食品矩阵中分离化合物,特别是汁.
  • 解决传统SPE的缺点,包括分析物的蒸发和减少回收,特别是对于挥发性和热敏化合物.

主要方法:

  • 设计和实施了一种新的固相提取 (SPE) 方法和专门的提取组件.
  • 开发的方法用于从果汁中提取化合物.
  • 提取的酸盐使用液态染色学高分辨率质谱学 (LC-HRMS) 系统进行了分析.

主要成果:

  • 开发的SPE方法和组装成功地消除了对蒸发步骤的需求.
  • 这有助于分析广泛的挥发性和非挥发性化合物,而不存在蒸发风险.
  • 与传统的SPE组件相比,该方法证明了效率和精度的提高,特别是对于热敏化合物,相比传统的SPE组件.

结论:

  • 创新的SPE技术和组装在食品分析样本准备方面取得了重大进展.
  • 它可以准确分析挥发性和热敏化合物,提高通过LC-HRMS获得的结果的可靠性.
  • 这种方法提供了一种更高效和有效的方法,用于从复杂的食品和饮料样本中分离活性成分.