三种不同的碳水化合物体外消化方法的比较
Yahao Xiao1, Sheng Li2, Jiaxi Li1
1College of Food Science and Technology, Henan University of Technology, Lianhua Road, Zhengzhou, 450001 China.
Journal of food science and technology
|January 27, 2025
概括
通过比较从消化大米中分析葡萄糖的三种方法,光谱仪方法证明了它适合在各种食品系统中进行准确和可重复的碳水化合物消化分析,提供灵敏度和精度的平衡.
科学领域:
- 食品科学 食品科学 食品科学
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 从消化的大米中量化释放的葡萄糖对于了解碳水化合物的生物可用性至关重要.
- 光谱仪,ELISA和HPAEC-PAD是常用的方法,但它们的可比性是不确定的.
- 调查方法的局限性,包括检测和量化限制,对于可靠的结果至关重要.
研究的目的:
- 为了比较光谱仪,ELISA和HPAEC-PAD的分析性能,用于量化体外大米消化过程中释放的葡萄糖.
- 评估每个方法的检测极限 (LOD) 和量化极限 (LOQ).
- 确定这些方法是否适合在各种食物矩阵中分析碳水化合物消化.
主要方法:
- 在体外消化大米,然后使用光谱仪,ELISA和高性能离子交换色谱与脉冲电压检测 (HPAEC-PAD) 进行分析.
- 评估每种方法的日内和日间精度,准确度 (恢复),LOD和LOQ.
- 应用所选择的方法来分析饼干和蒸面包中的碳水化合物消化.
主要成果:
- 所有方法都表现出良好的线性 (r2 ≥ 0.993).
- HPAEC-PAD提供了最低的LOD (0.07 mg/L) 和LOQ (0.24 mg/L),而ELISA则提供了最高的LOD (0.07 mg/L).
- 光谱相仪方法显示可接受的恢复 (98.62100.56%) 并被选择进行进一步分析,揭示了不同类型的食物中粉消化的差异.
结论:
- 光谱相仪方法提供了适合的灵敏度,精度和准确性的平衡,用于分析大米和其他食品系统中的碳水化合物消化.
- 尽管HPAEC-PAD具有很高的灵敏度,但可能会低估粉消化,而ELISA则缺乏某些应用所需的灵敏度.
- 建议采用光谱仪方法,用于复制碳水化合物消化分析复杂的食物矩阵,如饼干和蒸面包.
相关概念视频
Carbohydrate Digestion
113.6K
Carbohydrate digestion and metabolism break down simple and complex carbohydrates from food into saccharides (i.e., sugars) for the body to use as energy. Carbohydrate digestion starts in the mouth during mastication, or chewing. The masticated carbohydrates remain intact in the stomach. Digestion resumes in the duodenum of the small intestine, where pancreatic alpha-amylase and brush border enzymes of the microvilli convert complex carbohydrates to monosaccharides. Finally, the monosaccharides...
113.6K
Carbohydrate Metabolism
10.8K
Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
10.8K
Carbohydrate Absorption
463
Carbohydrates are essential macronutrients that serve as the body's primary energy source. Their digestion begins in the mouth, where salivary amylase partially breaks down complex carbohydrates such as starch into smaller oligosaccharides. This mechanical and enzymatic activity prepares carbohydrates for further processing in the gastrointestinal tract.
After being swallowed, the partially digested carbohydrates mix with gastric secretions in the stomach. However, the acidic environment...
After being swallowed, the partially digested carbohydrates mix with gastric secretions in the stomach. However, the acidic environment...
463
Introduction to Carbohydrates
12.2K
Carbohydrates, proteins, and fats are the primary macronutrients in the human diet. However, carbohydrates are the most favored source of energy in the body. They can be found in a wide variety of foods, including whole grains, fruit, and vegetables, in various forms, such as sugars, starch, and dietary fiber. Based on their structure, carbohydrates are classified into three main classes— monosaccharides, disaccharides, and polysaccharides. The body's cells can only utilize simple...
12.2K
Overview of Carbohydrate Metabolism
694
Carbohydrate metabolism is a fundamental biochemical process that ensures a constant supply of energy to living cells. The most important carbohydrate is glucose, which can be broken down via glycolysis to enter into the Krebs cycle and eventually lead to the production of ATP through oxidative phosphorylation.
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
Glucose transport into cells is facilitated by a family of transport proteins called GLUT (Glucose Transporters). GLUT4 is the primary glucose transporter for insulin-stimulated glucose...
694


