相关实验视频

Updated: May 30, 2025

Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum

Author Spotlight: THP-1 Macrophage Response to LPS/ATP — Unveiling the Pyroptosis, Apoptosis, and Necroptosis Spectrum

Published on: May 3, 2024

聚糖类通过激活自途径减轻巨细胞中LPS诱导的炎症反应

Manzeremu Rejiepu¹, Jun Shen¹, Junqing Liang¹

¹Clinical Medical Research Institute, the First Affiliated Hospital of Xinjiang Medical University, Xinjiang Medical University Urumqi Xinjiang China.

Food science & nutrition

|January 27, 2025

概括

佩根姆哈马拉多糖 (LTP) 通过AMPK-mTOR通路激活自,减少巨细胞中TNF-α和IL-6等炎症标志物. 这项研究揭示了LTP.

科学领域:

药理学和分子生物学
免疫学和炎症研究研究

背景情况:

佩加 (Peganum harmala) 是一个Zygophyllaceae家族的植物,具有抗炎性质.
通过何种精确的机制,Peganum harmala多糖 (LTP) 诱导自并减少炎症,尚不清楚.

研究的目的:

研究LTP在诱导自的作用.
评估LTP在缓解巨细胞炎症方面的有效性.
阐明涉及LTP介导自的信号通路.

主要方法:

使用GFP-LC3载体和共聚焦显微镜评估的自细胞形成.
西方涂抹用于分析与自相关的蛋白质 (LC3-II,p62) 和p70S6K酸化.
在实验室测定RAW264.7巨细胞,用脂多糖 (LPS) 刺激,通过西式涂抹和ELISA量化炎症标记物 (TNF-α,IL-6).

主要成果:

LTP显著增加了自活动,由巨细胞中LC3-II升高和p62水平降低表明.
由LTP诱导的自激活通过AMPK-mTOR信号通路发生.
在LPS刺激的巨细胞中,LTP治疗显著降低了促炎性细胞因子TNF-α和IL-6.

结论:

佩根姆 (Peganum) 的harmala多糖 (LTP) 有效地促进了巨细胞的自.
通过依赖mTOR的自诱导机制,LTP通过LPS刺激的巨细胞减少炎症.
在炎症性疾病中,LTP是潜在的治疗剂.

关键词:

在LPS中使用LPS.这是一种Peganum harmala多糖化合物.自自是自的过程.这是一种炎症炎症炎症炎症.在mTOROR中使用mTOR.巨细胞是什么?巨细胞是什么?

更多相关视频

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

A Macrophage Reporter Cell Assay to Examine Toll-Like Receptor-Mediated NF-kB/AP-1 Signaling on Adsorbed Protein Layers on Polymeric Surfaces

Published on: January 7, 2020

相关概念视频

Inflammatory Response

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...

Phagocytosis of Apoptotic Cells

Phagocytosis of Apoptotic Cells

Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...

Immune Surveillance by NK Cells and Phagocytes

Immune Surveillance by NK Cells and Phagocytes

Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...

Inflammation

Inflammation