在Podospora anserina中重建具有高内部保存的NOD类受体等位基因,使用长读测序
S Lorena Ament-Velásquez1, Brendan Furneaux2, Sonia Dheur3
1Department of Zoology, Stockholm University, 106 91 Stockholm, Sweden.
bioRxiv : the preprint server for biology
|January 27, 2025
概括
准确的真菌免疫受体WD40域的重建是可能的长读序列,揭示了关于异构细胞不兼容性和进化的见解.
科学领域:
- 免疫学 免疫学 免疫学
- 菌类学 菌类学是指菌类学.
- 遗传学 是一个遗传学.
背景情况:
- 类似NOD的受体 (NLR) 是细胞内免疫传感器.
- 在真菌中,NLRs调解异质细胞不兼容性,防止不同个体的融合.
- *Podospora anserina*中的多态het-d,het-e和het-c基因是这种识别系统的一个例子.
研究的目的:
- 准确地重建真菌NLRs的WD40域,用于功能和进化分析.
- 研究由het-d和het-e NLRs介导的异质核不相容的结构基础.
- 探索WD40重复域内的进化动态和序列变异性.
主要方法:
- 使用长读序列 (牛津纳米孔,PacBio) 进行准确的WD40域重建.
- 使用基于Illumina的组件进行比较.
- 生成WD40域的蛋白质结构模型.
主要成果:
- 长读序列精确地重建WD40域,与Illumina组件不同.
- 功能性等位基因通常包括11个保存的重复,组合的变化.
- 蛋白质模型显示了一个保存的7叶片β螺旋结构,具有神秘的分离重复.
- 一个特定的het-e等位基因,尽管其长度很长,但没有显示出不相容性.
结论:
- 准确的WD40域重建对于研究真菌NLRs至关重要.
- 重复的组合和神秘的重复有助于异质核不相容的特异性.
- 作为免疫传感器领域,WD40β螺旋表现出脆弱性和灵活性.
相关概念视频
Long-patch Base Excision Repair
7.0K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.0K
Exon Recombination
3.5K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.5K
RNA-seq
9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K
RACE - Rapid Amplification of cDNA Ends
6.3K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
6.3K


