对口腔微生物与宿主相互作用的生理相关的共同培养模型
Zeyang Pang1, Nicole Cady2, Lujia Cen3
1Department of Biomedical Engineering, College of Engineering and School of Medicine, University of Michigan, Ann Arbor, MI 48109-5622, USA.
bioRxiv : the preprint server for biology
|January 27, 2025
概括
一个新的不对称气体培系统精确地模仿了口腔微环境. 这种先进的模型增强了口腔细菌与宿主相互作用的研究,并改善了对口腔疾病抗生素治疗的评估.
科学领域:
- 微生物学 微生物学
- 口腔生物学 口腔生物学
- 细胞生物学 细胞生物学
背景情况:
- 了解口腔微生物与宿主相互作用对于口腔疾病病原和全身健康至关重要.
- 现有的体外培养模型不能复制口腔的生理氧度梯度.
- 这种限制阻碍了对无氧口腔细菌和宿主反应的准确研究.
研究的目的:
- 开发和验证一个模拟口腔微环境的不对称气体培系统.
- 为了研究生理氧度梯度对Fusobacterium核-牙上皮质细胞相互作用的影响.
- 评估系统对研究宿主反应和评估治疗干预措施的有用性.
主要方法:
- 设计了一个不对称的气体培系统,以保持不同的诺莫克斯和无氧条件.
- 在模拟的口腔条件下,用端粒酶不朽化的牙角质细胞与Fusobacterium nucleatum共同培养.
- 评估了细菌入侵,细胞内细菌负荷,宿主亲炎性细胞因子分泌和抗生素疗效.
主要成果:
- 该系统成功地保持了细菌活力和牙上皮细胞完整性.
- 与传统模型相比,不对称的系统显示了增强的细菌入侵和细胞内细菌负载.
- 观察到促炎性细胞因子 (CXCL10,IL-6,IL-8) 的分泌量增加,这表明宿主反应更强大.
- 该模型允许精确评估抗生素对细胞内口腔病原体的疗效.
结论:
- 非对称气体培系统为研究口腔微生物病原体提供了一个生理学上相关的平台.
- 这种模型增强了对口腔内无氧细菌与宿主相互作用的理解.
- 该系统对于查治疗药物和推进口腔和全身健康方面的研究非常有价值.
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