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相关概念视频

Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

592
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
592
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

227
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
227

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相关实验视频

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Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR
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在现场以光驱动的pH调制用于NMR研究.

Aarav Barde, Ruixian Han, Martin A Olson

    bioRxiv : the preprint server for biology
    |January 27, 2025
    PubMed
    概括

    研究人员开发了一种使用光激活光酸控制核磁共振 (NMR) 样本pH值的新方法. 这种非侵入性技术精确地调整pH值,以改善分子结构和功能的研究.

    科学领域:

    • 生物化学 生物化学
    • 分析化学 分析化学
    • 频谱学是一种光谱学.

    背景情况:

    • 质子交换对于化学事件至关重要,核磁共振 (NMR) 提供了特定地点的质子化解析.
    • 传统的NMR pH研究涉及手动定位,导致样本损失和信号减少.
    • 在NMR中控制pH值的现有方法是劳动密集型的,可能会损害样本的完整性.

    研究的目的:

    • 引入一种新的,非侵入性的方法,用于精确控制NMR样本中的pH值.
    • 为了证明水溶性光酸在in situ pH调节的使用.
    • 为了实现强大的和可靠的pH依赖的NMR研究,特别是生物分子.

    主要方法:

    • 使用水溶性光酸,在光照照射时释放质子以改变溶液pH.
    • 通过调整照明波长和强度来控制pH值.
    • 在现场使用内部标准监测pH值,并进行pH值敏感的化学变化.
    • 将该方法应用于具有显著缓冲容量 (>100微米) 的蛋白质样本.

    主要成果:

    • 在NMR磁体内实现了精确,校准和非侵入性的pH值变化.
    • 光诱导的pH控制有效调节了分子的质子化状态.

    更多相关视频

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    Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
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    相关实验视频

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    Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
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    Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
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  • 该方法显著减少了样品处理和潜在的样品损失.
  • 即使在具有相当大的缓冲能力的样品中,也证明了成功的pH调整.

    • 结论:

    • 使用光酸的光诱导pH控制为NMR中常规定位方法提供了强大的替代方案.
    • 这种方法提高了pH依赖的NMR研究的稳定性和可靠性.
    • 该技术在研究分子结构和功能方面具有广泛的应用,pH在其中发挥着关键作用.