30色纵向全谱免疫表型和分类人类循环免疫细胞子组涉及系统性自身免疫性风湿性疾病
bioRxiv : the preprint server for biology
|January 27, 2025
概括
这项研究引入了30色流细胞计面板,用于在自身免疫性疾病中精确的免疫细胞子集分析. 它可以从有限的样本中有效地分类和高参数免疫型化关键免疫细胞.
科学领域:
- 免疫学 免疫学 免疫学
- 自免疫性疾病 自免疫性疾病
- 流动细胞计量 (Flow Cytometry) 是一种流动细胞计.
背景情况:
- 系统性自身免疫性疾病,如类风湿性关节炎和狼,涉及复杂的免疫细胞失调.
- 准确的清单和免疫细胞子集的净化对于理解病变发生至关重要.
- 现有的方法可能缺乏全面免疫分析的解决方案或效率.
研究的目的:
- 开发和验证30色流细胞计面板,用于详细的免疫细胞子集分析.
- 为了促进在自身免疫性疾病中涉及的循环免疫细胞的清单和净化.
- 从有限的样本数量中提供一个强大的工具,用于从有限的样本数量中进行高参数免疫型定型.
主要方法:
- 开发一个使用30种标记物的30种颜色流细胞计面板.
- 使用CD45和自光光谱签名对外周血液单核细胞和其他组织类型的应用.
- 分析T细胞子集 (18种标记物),B细胞子集 (>10种标记物) 和免疫系 (8种标记物).
主要成果:
- 该面板可复制地识别出不同的免疫细胞群体,具有出色的分辨率.
- 在数据采集的几个月内,观察到最小的批量效应.
- 该面板可以有效地对免疫细胞子集进行分类,用于下游应用.
结论:
- 这个30种颜色的面板提供了一种实用且高分辨率的方法,用于自身免疫性疾病研究中的免疫细胞子集分析.
- 它允许同时进行高参数免疫型和细胞分类,从而保存有价值的样本材料.
- 该小组适用于调查免疫细胞参与系统性自身免疫性疾病及其他疾病.
相关概念视频
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Overview
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Overview
Defense Mechanism Against Infection
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In addition, many body organ systems have unique defenses against infection. The skin is an intact, multilayered surface preventing invasion by microorganisms unless impaired. Mucous membranes lining the mouth, nose, and eyelids are barriers...
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Classification of Epithelial Tissues: Overview
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Based on the number of cell layers,...
Based on the number of cell layers,...
Classification of Leukocytes
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Neutrophils are the most abundant type of granular leukocytes, comprising 50-70% of all leukocytes. They feature small, evenly distributed granules and a...
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Lymphoid Cells and Tissues
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
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