中性粒细胞对Streptococcus oralis生物膜体外的双向作用
Basmah M Almaarik1,2, Rizwan Ali3, Paul R Cooper4
1Clinical Laboratory Science Department (CLS), College of Applied Medical Sciences (CAMS), King Saud University (KSU), Riyadh, Saudi Arabia.
Journal of oral microbiology
|January 27, 2025
概括
中性粒细胞可以通过干扰细胞外DNA (eDNA) 来暂时减少Streptococcus oralis生物膜,尽管它们在没有形成中性粒细胞外陷 (NET) 的情况下意外增加了细菌数量.
科学领域:
- 口腔微生物学 口腔微生物学
- 这是天生的免疫力.
- 生物膜的动力学
背景情况:
- 口腔白斑菌 (Streptococcus oralis) 是一种常见的口腔细菌,也是早期生物膜殖民者.
- 细菌细胞外DNA (eDNA) 是生物膜矩阵的关键组成部分.
- 中性粒细胞是与生俱来的免疫细胞,它们使用诸如中性粒细胞外陷 (NETs) 和活性氧物种 (ROS) 等机制对抗生物膜.
研究的目的:
- 研究中性粒细胞对Streptococcus oralis生物膜的双向作用.
- 了解中性粒细胞如何影响生物膜数量,结构和细菌活力.
主要方法:
- 在不同发育阶段,中性粒细胞与S. oralis生物膜共同培养.
- 使用水晶紫色染色,共聚焦显微镜和CFU计数来测量生物膜数量.
- 细胞外DNA,ROS产生和中性粒细胞-eDNA相互作用使用光和发光测定,流细胞计和显微镜进行了分析.
主要成果:
- 中性粒细胞暂时降低了S. oralis生物膜数量,并降低了eDNA水平.
- 在中性粒细胞的存在下,细菌殖民地形成单位 (CFU) 意外增加.
- 中性粒细胞表现出增加的ROS产量,但在对生物膜的反应中没有形成NET.
- 观察到细菌DNA与中性粒细胞膜共同定位.
结论:
- 在体外,中性粒细胞不会被S. oralis生物膜过度激活.
- 中性粒细胞可以在短期内减少生物膜数量,可能是通过与eDNA相互作用.
- 这项研究突出了一个复杂的相互作用,中性粒细胞影响生物膜结构和细菌负载.
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