可光激活的植物激素基近距离化学诱导剂用于In Vivo应用
Philipp Pöschko1,2, Caroline M Berrou1, Kaisa Pakari3,4
1Department of Chemical Biology, Max Planck Institute for Medical Research, Jahnstraße 29, 69120 Heidelberg, Germany.
ACS chemical biology
|January 27, 2025
概括
研究人员开发了新的近距离化学诱导剂 (CIPs),以精确控制蛋白相互作用. 这些工具,pMandi和opabactin (OP),可以通过光激活细胞和胚胎中的蛋白质邻近的操纵.
科学领域:
- 生物化学 生化学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 蛋白质相互作用对细胞功能至关重要.
- 控制蛋白相互作用对于理解和操纵细胞机制至关重要.
- 接近的化学诱导剂 (CIPs) 提供了一种针对蛋白相互作用的有针对性的操纵方法.
研究的目的:
- 为了引入pMandi,一个Mandipropamid (Mandi) 的光版本,用于哺乳动物细胞中的光诱导蛋白质接近.
- 作为一种基于植物激素的新型,强效的CIP,用于哺乳动物细胞和medaka胚胎.
- 开发pOP,OP的光衍生物,用于单细胞分辨率和蛋白质近距离的时空控制.
主要方法:
- 开发和应用近距离化学诱导剂 (CIPs).
- 利用光作为外部触发器来诱导活哺乳动物细胞中的蛋白质接近.
- 测试新的CIPs (OP) 和它们的光衍生物 (pOP) 在活体medaka胚胎中的疗效.
主要成果:
- 在活哺乳动物细胞中,pMandi可实现光控制的蛋白质接近.
- 在低度下, Opabactin (OP) 在哺乳动物细胞和 medaka 胚胎中显示出高疗效.
- 照相的奥巴巴丁 (pOP) 允许在单细胞水平上精确的,光感应的蛋白质接近.
- 在活体Medaka胚胎中成功应用光CIP.
结论:
- pMandi 和 opabactin (OP) 代表了控制蛋白质与蛋白质相互作用的先进工具.
- 照片的CIP提供了前所未有的时空控制,直到单细胞分辨率.
- 这些新型化学工具在细胞生物学和体内发育研究中具有广泛的应用.
相关概念视频
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
01:56Plant Hormones
23.4K
Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.
23.4K
02:00Photoreceptors and Plant Responses to Light
20.1K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
20.1K
02:50Transgenic Plants
7.1K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.1K